CPLM-009536

Genbank Nucleotide ID

Rho-related GTP-binding protein RhoB

Protein Synonyms/Alias

Rho cDNA clone 6; h6

Homo sapiens (Human)

Position	Peptide	Type	References
135	RTELARMKQEPVRTD	ubiquitination	[1, 2]

[1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.

NP_BIND 12 19 GTP (By similarity).
NP_BIND 59 63 GTP (By similarity).
NP_BIND 117 120 GTP (By similarity).
MOTIF 34 42 Effector region (Potential).
MOD_RES 41 41 ADP-ribosylasparagine; by botulinum toxin
MOD_RES 154 154 Phosphotyrosine (By similarity).
MOD_RES 193 193 Cysteine methyl ester.
LIPID 189 189 S-palmitoyl cysteine.
LIPID 192 192 S-palmitoyl cysteine.
LIPID 193 193 S-farnesyl cysteine; in plasma membrane
LIPID 193 193 S-geranylgeranyl cysteine; in endosomal

3D-structure; ADP-ribosylation; Angiogenesis; Apoptosis; Cell adhesion; Cell membrane; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Endosome; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Nucleus; Palmitate; Phosphoprotein; Prenylation; Protein transport; Reference proteome; Transport; Tumor suppressor.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0032154; C:cleavage furrow; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0010008; C:endosome membrane; IDA:UniProtKB.
GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0005886; C:plasma membrane; IDA:UniProtKB.
GO:0019003; F:GDP binding; IEA:Compara.
GO:0005525; F:GTP binding; TAS:UniProtKB.
GO:0003924; F:GTPase activity; TAS:UniProtKB.
GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
GO:0007411; P:axon guidance; TAS:Reactome.
GO:0007155; P:cell adhesion; ISS:UniProtKB.
GO:0070301; P:cellular response to hydrogen peroxide; IDA:UniProtKB.
GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB.
GO:0000910; P:cytokinesis; IMP:UniProtKB.
GO:0008333; P:endosome to lysosome transport; IDA:UniProtKB.
GO:0045786; P:negative regulation of cell cycle; ISS:UniProtKB.
GO:0030168; P:platelet activation; TAS:Reactome.
GO:0045766; P:positive regulation of angiogenesis; ISS:UniProtKB.
GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
GO:0007266; P:Rho protein signal transduction; TAS:UniProtKB.
GO:0006927; P:transformed cell apoptotic process; ISS:UniProtKB.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR003578; Small_GTPase_Rho.

PR00449; RASTRNSFRMNG.