CPLM-022850

Genbank Nucleotide ID

Eukaryotic translation initiation factor 3 subunit K

Protein Synonyms/Alias

eIF3k; Eukaryotic translation initiation factor 3 subunit 12; Muscle-specific gene M9 protein; PLAC-24; eIF-3 p25; eIF-3 p28

EIF3S12; ARG134; HSPC029; MSTP001; PTD001

Homo sapiens (Human)

Position	Peptide	Type	References
13	QMRANVGKLLKGIDR	ubiquitination	[1, 2, 3, 4, 5, 6]
16	ANVGKLLKGIDRYNP	ubiquitination	[1, 2, 3, 4, 5, 6]
38	RYVETQAKENAYDLE	ubiquitination	[1, 2, 5, 6, 7]
140	GFEDSVRKFICHVVG	ubiquitination	[8]
197	CSQEESIKPKNIVEK	ubiquitination	[5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

MOD_RES 2 2 N-acetylalanine.
MOD_RES 217 217 Phosphoserine.

3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Initiation factor; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:HPA.
GO:0043022; F:ribosome binding; IEA:InterPro.
GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
GO:0006413; P:translational initiation; IDA:UniProtKB.

IPR016024; ARM-type_fold.
IPR009374; eIF3k.
IPR016020; Transl_init_fac_sub12_N_euk.
IPR011991; WHTH_DNA-bd_dom.