UniProt Accession

 HBS1L_HUMAN; Q9Y450; B7Z365; Q4VX89; Q4VX90; Q5T7G3; Q8NDW9; Q9UPW3 

Genbank Protein ID

 U87791; AJ459826; AJ459827; AK295545; AL353596; AL445190; AL353596; AL445190; AL353596; AL445190; AL353596; AL445190; AL353596; CH471051; BC001465; BC040849; AB028961 

Genbank Nucleotide ID

 AAD00645.1; CAD30873.1; CAD30874.1; BAH12101.1; CAI17912.1; CAI17912.1; CAI17913.1; CAI17913.1; CAI17914.1; CAI95161.1; CAI95161.1; CAI95162.1; CAI95162.1; EAW47982.1; AAH01465.1; AAH40849.1; BAA82990.1 

Protein Name

 HBS1-like protein 

Protein Synonyms/Alias

 ERFS 

Gene Name

 HBS1L 

Gene Synonyms/Alias

 HBS1; KIAA1038 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	FIYSRRDKPSVEPVE	ubiquitination	[1, 2]
62	EYDYEDLKESSNSVS	ubiquitination	[1, 3]
107	ILIEAVLKNKFDVQK	ubiquitination	[1]
114	KNKFDVQKALSGVLE	ubiquitination	[1, 4]
140	EATVSTGKIAKGKPV	ubiquitination	[1]
237	STPAPVKKSGKLRQQ	ubiquitination	[2]
332	TMDVGMTKFETTTKV	ubiquitination	[2]
424	RFQEITGKLGHFLKQ	ubiquitination	[2]
430	GKLGHFLKQAGFKES	ubiquitination	[2]
435	FLKQAGFKESDVGFI	ubiquitination	[2]
463	SQSSELTKWYKGLCL	ubiquitination	[2]
466	SELTKWYKGLCLLEQ	ubiquitination	[2]
478	LEQIDSFKPPQRSID	ubiquitination	[2]
486	PPQRSIDKPFRLCVS	ubiquitination	[2]
497	LCVSDVFKDQGSGFC	ubiquitination	[2]
572	GCIFCGPKVPIKACT	ubiquitination	[2]
622	RLISVLNKSTGEVTK	ubiquitination	[2, 4]
657	PIALELYKDFKELGR	ubiquitination	[4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

 NP_BIND 267 274 GTP (By similarity).
 NP_BIND 344 348 GTP (By similarity).
 NP_BIND 406 409 GTP (By similarity).
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 127 127 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Elongation factor; GTP-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 684 AA 

Protein Sequence

Gene Ontology

 GO:0005525; F:GTP binding; TAS:ProtInc.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0006412; P:translation; TAS:ProtInc. 

Interpro

 IPR015033; DUF1916.
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 

Pfam

 PF08938; DUF1916
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3 

SMART

  

PROSITE

 PS00301; EFACTOR_GTP 

PRINTS

 PR00315; ELONGATNFCT.