CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030990
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA topoisomerase 2-beta 
Protein Synonyms/Alias
 cDNA FLJ50986, highly similar to DNA topoisomerase 2-beta (EC 5.99.1.3) 
Gene Name
 TOP2B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66VLAGMSGKAIKGKVGacetylation[1]
66VLAGMSGKAIKGKVGubiquitination[2]
79VGKPKVKKLQLEETMubiquitination[2, 3, 4]
102IPEITAMKADASKKLubiquitination[2, 3, 4]
107AMKADASKKLLKKKKubiquitination[5]
114KKLLKKKKGDLDTAAubiquitination[2]
151TPSVPINKGPKPKREubiquitination[2]
154VPINKGPKPKREKKEubiquitination[2]
175KTPTSSGKPSAKKVKacetylation[4]
175KTPTSSGKPSAKKVKubiquitination[2]
286KSKATPEKSLHDKKSacetylation[4]
292EKSLHDKKSQDFGNLubiquitination[3]
338FGLKQTDKVPSKTVAacetylation[4]
426TTSKKPKKTSFDQDSubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Isomerase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 478 AA 
Protein Sequence
MSLWSLTKEK VEELIKQRDA KGREVNDLKR KSPSDLWKED LAAFVEELDK VESQEREDVL 60
AGMSGKAIKG KVGKPKVKKL QLEETMPSPY GRRIIPEITA MKADASKKLL KKKKGDLDTA 120
AVKVEFDEEF SGAPVEGAGE EALTPSVPIN KGPKPKREKK EPGTRVRKTP TSSGKPSAKK 180
VKKRNPWSDD ESKSESDLEE TEPVVIPRDS LLRRAAAERP KYTFDFSEEE DDDADDDDDD 240
NNDLEELKVK ASPITNDGED EFVPSDGLDK DEYTFSPGKS KATPEKSLHD KKSQDFGNLF 300
SFPSYSQKSE DDSAKFDSNE EDSASVFSPS FGLKQTDKVP SKTVAAKKGK PSSDTVPKPK 360
RAPKQKKVVE AVNSDSDSEF GIPKKTTTPK GKGRGAKKRK ASGSENEGDY NPGRKTSKTT 420
SKKPKKTSFD QDSDVDIFPS DFPTEPPSLP RTGRARKEVK YFAESDEEED DVDFAMFN 478 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003918; F:DNA topoisomerase type II (ATP-hydrolyzing) activity; IEA:InterPro.
 GO:0007409; P:axonogenesis; IEA:Compara.
 GO:0006265; P:DNA topological change; IEA:InterPro.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara. 
Interpro
 IPR012542; DTHCT.
 IPR013757; Topo_IIA_A_a.
 IPR013760; Topo_IIA_like_dom. 
Pfam
 PF08070; DTHCT 
SMART
  
PROSITE
  
PRINTS