CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018734
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nicastrin 
Protein Synonyms/Alias
  
Gene Name
 NCSTN 
Gene Synonyms/Alias
 KIAA0253; UNQ1874/PRO4317 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
331VYDMEKGKFPVQLENubiquitination[1]
383DLLATLEKSGAGVPAubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. 
Sequence Annotation
 CARBOHYD 45 45 N-linked (GlcNAc...).
 CARBOHYD 55 55 N-linked (GlcNAc...) (Potential).
 CARBOHYD 187 187 N-linked (GlcNAc...).
 CARBOHYD 200 200 N-linked (GlcNAc...) (Potential).
 CARBOHYD 204 204 N-linked (GlcNAc...) (Potential).
 CARBOHYD 264 264 N-linked (GlcNAc...) (Potential).
 CARBOHYD 387 387 N-linked (GlcNAc...).
 CARBOHYD 417 417 N-linked (GlcNAc...) (Potential).
 CARBOHYD 435 435 N-linked (GlcNAc...) (Potential).
 CARBOHYD 464 464 N-linked (GlcNAc...) (Potential).
 CARBOHYD 506 506 N-linked (GlcNAc...) (Potential).
 CARBOHYD 530 530 N-linked (GlcNAc...) (Potential).
 CARBOHYD 562 562 N-linked (GlcNAc...) (Potential).
 CARBOHYD 573 573 N-linked (GlcNAc...) (Potential).
 CARBOHYD 580 580 N-linked (GlcNAc...) (Potential).
 CARBOHYD 612 612 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Complete proteome; Disease mutation; Glycoprotein; Membrane; Notch signaling pathway; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 709 AA 
Protein Sequence
MDFNLILESL CRGNSVERKI YIPLNKTAPC VRLLNATHQI GCQSSISGDT GVIHVVEKEE 60
DLQWVLTDGP NPPYMVLLES KHFTRDLMEK LKGRTSRIAG LAVSLTKPSP ASGFSPSVQC 120
PNDGFGVYSN SYGPEFAHCR EIQWNSLGNG LAYEDFSFPI FLLEDENETK VIKQCYQDHN 180
LSQNGSAPTF PLCAMQLFSH MHAVISTATC MRRSSIQSTF SINPEIVCDP LSDYNVWSML 240
KPINTTGTLK PDDRVVVAAT RLDSRSFFWN VAPGAESAVA SFVTQLAAAE ALQKAPDVTT 300
LPRNVMFVFF QGETFDYIGS SRMVYDMEKG KFPVQLENVD SFVELGQVAL RTSLELWMHT 360
DPVSQKNESV RNQVEDLLAT LEKSGAGVPA VILRRPNQSQ PLPPSSLQRF LRARNISGVV 420
LADHSGAFHN KYYQSIYDTA ENINVSYPEW LSPEEDLNFV TDTAKALADV ATVLGRALYE 480
LAGGTNFSDT VQADPQTVTR LLYGFLIKAN NSWFQSILRQ DLRSYLGDGP LQHYIAVSSP 540
TNTTYVVQYA LANLTGTVVN LTREQCQDPS KVPSENKDLY EYSWVQGPLH SNETDRLPRC 600
VRSTARLARA LSPAFELSQW SSTEYSTWTE SRWKDIRARI FLIASKELEL ITLTVGFGIL 660
IFSLIVTYCI NAKADVLFIA PREPGAVSY 689 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0005887; C:integral to plasma membrane; ISS:UniProtKB.
 GO:0005765; C:lysosomal membrane; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0004175; F:endopeptidase activity; IEA:Compara.
 GO:0042987; P:amyloid precursor protein catabolic process; TAS:HGNC.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0050435; P:beta-amyloid metabolic process; IEA:Compara.
 GO:0050673; P:epithelial cell proliferation; IEA:Compara.
 GO:0006509; P:membrane protein ectodomain proteolysis; ISS:UniProtKB.
 GO:0031293; P:membrane protein intracellular domain proteolysis; TAS:Reactome.
 GO:0002262; P:myeloid cell homeostasis; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007220; P:Notch receptor processing; TAS:HGNC.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0043085; P:positive regulation of catalytic activity; ISS:UniProtKB.
 GO:0016485; P:protein processing; ISS:UniProtKB.
 GO:0042098; P:T cell proliferation; IEA:Compara. 
Interpro
 IPR008710; Nicastrin. 
Pfam
 PF05450; Nicastrin 
SMART
  
PROSITE
  
PRINTS