UniProt Accession

 TCEA1_MOUSE; P10711; P10712; P23713 

Genbank Protein ID

 M18209; M18210; D00925; BC006022; BC061490; BC083127 

Genbank Nucleotide ID

 AAA40418.1; AAA40419.1; BAA00768.1; AAH06022.1; AAH61490.1; AAH83127.1 

Protein Name

 Transcription elongation factor A protein 1 

Protein Synonyms/Alias

 Transcription elongation factor S-II protein 1; Transcription elongation factor TFIIS.o 

Gene Name

 Tcea1 

Gene Synonyms/Alias

 Tceat 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
14	RIAKKMDKMVQKKNA	acetylation	[1]
29	AGALDLLKELKNIPM	ubiquitination	[2]
32	LDLLKELKNIPMTLE	ubiquitination	[2]
67	EEVTSLAKSLIKSWK	ubiquitination	[2]
234	EMASDELKEMRKNLT	ubiquitination	[2]
242	EMRKNLTKEAIREHQ	ubiquitination	[2]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. 

Sequence Annotation

 DOMAIN 3 80 TFIIS N-terminal.
 DOMAIN 140 256 TFIIS central.
 ZN_FING 259 299 TFIIS-type.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 97 97 Phosphoserine.
 MOD_RES 100 100 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030218; P:erythrocyte differentiation; IMP:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0032784; P:regulation of DNA-dependent transcription, elongation; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR016492; TF_IIS-rel.
 IPR003617; TFIIS/CRSP70_N_sub.
 IPR003618; TFIIS_cen_dom.
 IPR017923; TFIIS_N.
 IPR017890; TFS2M.
 IPR006289; TFSII.
 IPR001222; Znf_TFIIS. 

Pfam

 PF08711; Med26
 PF01096; TFIIS_C
 PF07500; TFIIS_M 

SMART

 SM00510; TFS2M
 SM00509; TFS2N
 SM00440; ZnF_C2C2 

PROSITE

 PS51321; TFIIS_CENTRAL
 PS51319; TFIIS_N
 PS00466; ZF_TFIIS_1
 PS51133; ZF_TFIIS_2 

PRINTS