UniProt Accession

 5NTD_HUMAN; P21589; B3KQI8; O75520; Q5W116 

Genbank Protein ID

 X55740; AK075008; AL135903; AL589666; AL589666; AL135903; U21730; AF069067 

Genbank Nucleotide ID

 CAA39271.1; BAG52050.1; CAH72337.1; CAH72337.1; CAI40168.1; CAI40168.1; AAA96950.1; AAC98672.1 

Protein Name

 5'-nucleotidase 

Protein Synonyms/Alias

 5'-NT; Ecto-5'-nucleotidase; CD73 

Gene Name

 NT5E 

Gene Synonyms/Alias

 NT5; NTE 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	QTSEDSSKCVNASRC	ubiquitination	[1]
133	GLIEPLLKEAKFPIL	ubiquitination	[1]
206	ALQPEVDKLKTLNVN	ubiquitination	[1]
262	SKEVPAGKYPFIVTS	ubiquitination	[1]
321	IPEDPSIKADINKWR	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. 

Sequence Annotation

 REGION 500 506 Substrate binding.
 METAL 36 36 Zinc 1.
 METAL 38 38 Zinc 1.
 METAL 85 85 Zinc 1.
 METAL 85 85 Zinc 2.
 METAL 117 117 Zinc 2.
 METAL 220 220 Zinc 2.
 METAL 243 243 Zinc 2.
 BINDING 245 245 Substrate.
 BINDING 354 354 Substrate.
 BINDING 390 390 Substrate.
 BINDING 395 395 Substrate.
 BINDING 417 417 Substrate.
 LIPID 549 549 GPI-anchor amidated serine.
 CARBOHYD 53 53 N-linked (GlcNAc...) (Potential).
 CARBOHYD 311 311 N-linked (GlcNAc...).
 CARBOHYD 333 333 N-linked (GlcNAc...).
 CARBOHYD 403 403 N-linked (GlcNAc...).
 DISULFID 51 57
 DISULFID 353 358
 DISULFID 365 387
 DISULFID 476 479  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Membrane; Metal-binding; Nucleotide-binding; Polymorphism; Reference proteome; Signal; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 574 AA 

Protein Sequence

Gene Ontology

 GO:0031225; C:anchored to membrane; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0008253; F:5'-nucleotidase activity; EXP:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0046086; P:adenosine biosynthetic process; IEA:Compara.
 GO:0006196; P:AMP catabolic process; IEA:Compara.
 GO:0006259; P:DNA metabolic process; TAS:ProtInc.
 GO:0050728; P:negative regulation of inflammatory response; IEA:Compara.
 GO:0006144; P:purine nucleobase metabolic process; TAS:Reactome.
 GO:0006195; P:purine nucleotide catabolic process; TAS:Reactome.
 GO:0006206; P:pyrimidine nucleobase metabolic process; TAS:Reactome.
 GO:0046135; P:pyrimidine nucleoside catabolic process; TAS:Reactome. 

Interpro

 IPR008334; 5'-Nucleotdase_C.
 IPR006146; 5'-Nucleotdase_CS.
 IPR006179; 5_nucleotidase/apyrase.
 IPR004843; Metallo_PEstase_dom. 

Pfam

 PF02872; 5_nucleotid_C
 PF00149; Metallophos 

SMART

  

PROSITE

 PS00785; 5_NUCLEOTIDASE_1
 PS00786; 5_NUCLEOTIDASE_2 

PRINTS

 PR01607; APYRASEFAMLY.