UniProt Accession

 BAAT_RAT; Q63276; Q5M8A2 

Genbank Protein ID

 D43964; BC088153 

Genbank Nucleotide ID

 BAA07901.1; AAH88153.1 

Protein Name

 Bile acid-CoA:amino acid N-acyltransferase 

Protein Synonyms/Alias

 BACAT; BAT; Glycine N-choloyltransferase; Kan-1; Long-chain fatty-acyl-CoA hydrolase 

Gene Name

 Baat 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKLTAVPLS	acetylation	[1]
40	QASLKDDKGNLFNSQ	acetylation	[1]
305	TFEETADKDSKYCFP	acetylation	[1]
346	QAIAQLMKSGKKNWT	acetylation	[1]
406	HSWKEIQKFLKQHLN	acetylation	[1]
409	KEIQKFLKQHLNPGF	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs (By similarity). 

Sequence Annotation

 ACT_SITE 235 235 Charge relay system (By similarity).
 ACT_SITE 328 328 Charge relay system (By similarity).
 ACT_SITE 362 362 Charge relay system (By similarity).  

Keyword

 Acyltransferase; Complete proteome; Fatty acid metabolism; Hydrolase; Lipid metabolism; Peroxisome; Reference proteome; Serine esterase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 420 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005777; C:peroxisome; IDA:HGNC.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0047963; F:glycine N-choloyltransferase activity; IEA:EC.
 GO:0052815; F:medium-chain acyl-CoA hydrolase activity; IEA:Compara.
 GO:0016410; F:N-acyltransferase activity; IEA:Compara.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IEA:EC.
 GO:0052817; F:very long chain acyl-CoA hydrolase activity; IEA:Compara.
 GO:0006637; P:acyl-CoA metabolic process; IEA:Compara.
 GO:0006699; P:bile acid biosynthetic process; IEA:Compara.
 GO:0002152; P:bile acid conjugation; IEA:Compara.
 GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
 GO:0006544; P:glycine metabolic process; IEA:Compara.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0031100; P:organ regeneration; IEP:RGD.
 GO:0019530; P:taurine metabolic process; IEA:Compara. 

Interpro

 IPR016662; Acyl-CoA_thioEstase_long-chain.
 IPR014940; BAAT_C.
 IPR006862; Thio_Ohase/aa_AcTrfase. 

Pfam

 PF08840; BAAT_C
 PF04775; Bile_Hydr_Trans 

SMART

  

PROSITE

  

PRINTS