| Tag | Content |
|---|
| CPLM ID | CPLM-002548 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Cell division control protein 4 |
Protein Synonyms/Alias | E3 ubiquitin ligase complex SCF subunit CDC4; F-box protein CDC4 |
Gene Name | CDC4 |
Gene Synonyms/Alias | YFL009W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 202 | TPLAKTTKTINNNNN | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. Directs ubiquitination of the phosphorylated CDK inhibitor SIC1. Involved in the degradation of CDC6 together with CDC34/UBC3 and CDC53, and in restricting the degradation of FAR1 to the nucleus. Is essential for initiation of DNA replication and separation of the spindle pole bodies to form the poles of the mitotic spindle. It also plays a role in bud development, fusion of zygotic nuclei after conjugation and various aspects of sporulation. Required for HTA1-HTB1 locus transcription activation. Required for G1/S and G2/M transition. |
Sequence Annotation | DOMAIN 272 319 F-box.
REPEAT 380 408 WD 1.
REPEAT 420 449 WD 2.
REPEAT 461 493 WD 3.
REPEAT 528 556 WD 4.
REPEAT 568 598 WD 5.
REPEAT 630 658 WD 6.
REPEAT 669 698 WD 7.
MOTIF 82 85 Nuclear localization signal.
MOD_RES 104 104 Phosphoserine. |
Keyword | 3D-structure; Cell cycle; Cell division; Complete proteome; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Sporulation; Ubl conjugation pathway; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 779 AA |
Protein Sequence | MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT VETEDGEEDI 60
DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT GAASVDNDGL HNLTDISNDA 120
EKLLMSVDDG SAAPSTLSVN MGVASHNVAA PTTVNAATIT GSDVSNNVNS ATINNPMEEG 180
ALPLSPTASS PGTTTPLAKT TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH 240
AYFKNLLFRL VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG 300
VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR LRLSFLENIF 360
ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD DKMIRVYDSI NKKFLLQLSG 420
HDGGVWALKY AHGGILVSGS TDRTVRVWDI KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI 480
VTGSRDNTLH VWKLPKESSV PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG 540
NIVVSGSYDN TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE 600
NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA DGSIRGWDAN 660
DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR SGKLVHANIL KDADQIWSVN 720
FKGKTLVAAV EKDGQSFLEI LDFSKASKIN YVSNPVNSSS SSLESISTSL GLTRTTIIP 779 |
Gene Ontology | GO:0016363; C:nuclear matrix; IDA:SGD. GO:0043224; C:nuclear SCF ubiquitin ligase complex; IDA:SGD. GO:0043130; F:ubiquitin binding; IDA:SGD. GO:0051301; P:cell division; IEA:UniProtKB-KW. GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:SGD. GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD. GO:0007126; P:meiosis; IMP:SGD. GO:0007067; P:mitosis; IEA:UniProtKB-KW. GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:SGD. GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD. GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. |
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