CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018818
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein ubi-d4 
Protein Synonyms/Alias
 Apoptosis response zinc finger protein; BRG1-associated factor 45D; BAF45D; D4, zinc and double PHD fingers family 2; Protein requiem 
Gene Name
 DPF2 
Gene Synonyms/Alias
 BAF45D; REQ; UBID4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59NCYIWMEKRHRGPGLubiquitination[1]
108DTDQTLKKEGLISQDubiquitination[1, 2, 3, 4]
130LRTDPLEKRGAPDPRubiquitination[1]
285GDSKINKKTGQPEELubiquitination[1]
381CLDLLKEKASIYQNQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells. 
Sequence Annotation
 ZN_FING 209 232 C2H2-type.
 ZN_FING 270 330 PHD-type 1.
 ZN_FING 327 377 PHD-type 2.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 142 142 Phosphoserine.
 MOD_RES 172 172 Phosphotyrosine.
 MOD_RES 176 176 Phosphothreonine.
 MOD_RES 200 200 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 248 248 Phosphothreonine (By similarity).  
Keyword
 3D-structure; Acetylation; Apoptosis; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 391 AA 
Protein Sequence
MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ SNCYIWMEKR 60
HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP DTDQTLKKEG LISQDGSSLE 120
ALLRTDPLEK RGAPDPRVDD DSLGEFPVTN SRARKRILEP DDFLDDLDDE DYEEDTPKRR 180
GKGKSKGKGV GSARKKLDAS ILEDRDKPYA CDICGKRYKN RPGLSYHYAH SHLAEEEGED 240
KEDSQPPTPV SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR 300
SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NICGTSENDD QLLFCDDCDR GYHMYCLTPS 360
MSEPPEGSWS CHLCLDLLKE KASIYQNQNS S 391 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:ProtInc.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR025750; Requiem/DPF_N_dom.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR019787; Znf_PHD-finger.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00628; PHD
 PF14051; Requiem_N 
SMART
 SM00249; PHD
 SM00184; RING
 SM00355; ZnF_C2H2 
PROSITE
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS