CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023073
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lariat debranching enzyme 
Protein Synonyms/Alias
  
Gene Name
 DBR1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
128GGISGIFKSHDYRKGacetylation[1, 2]
250GQTARATKFLALDKCubiquitination[3]
492STIDREGKPGGTVESubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via a RNA lariat intermediate in cDNA synthesis. 
Sequence Annotation
 MOD_RES 128 128 N6-acetyllysine.
 MOD_RES 474 474 Phosphoserine.
 MOD_RES 478 478 Phosphoserine.
 MOD_RES 479 479 Phosphoserine.
 MOD_RES 499 499 Phosphoserine (By similarity).
 MOD_RES 514 514 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Hydrolase; Metal-binding; mRNA processing; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 544 AA 
Protein Sequence
MRVAVAGCCH GELDKIYETL ALAERRGPGP VDLLLCCGDF QAVRNEADLR CMAVPPKYRH 60
MQTFYRYYSG EKKAPVLTLF IGGNHEASNH LQELPYGGWV APNIYYLGLA GVVKYRGVRI 120
GGISGIFKSH DYRKGHFECP PYNSSTIRSI YHVRNIEVYK LKQLKQPIDI FLSHDWPRSI 180
YHYGNKKQLL KTKSFFRQEV ENNTLGSPAA SELLEHLKPT YWFSAHLHVK FAALMQHQAK 240
DKGQTARATK FLALDKCLPH RDFLQILEIE HDPSAPDYLE YDIEWLTILR ATDDLINVTG 300
RLWNMPENNG LHARWDYSAT EEGMKEVLEK LNHDLKVPCN FSVTAACYDP SKPQTQMQLI 360
HRINPQTTEF CAQLGIIDIN VRLQKSKEEH HVCGEYEEQD DVESNDSGED QSEYNTDTSA 420
LSSINPDEIM LDEEEDEDSI VSAHSGMNTP SVEPSDQASE FSASFSDVRI LPGSMIVSSD 480
DTVDSTIDRE GKPGGTVESG NGEDLTKVPL KRLSDEHEPE QRKKIKRRNQ AIYAAVDDDD 540
DDAA 544 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008419; F:RNA lariat debranching enzyme activity; IMP:UniProtKB.
 GO:0000398; P:mRNA splicing, via spliceosome; IEA:Compara.
 GO:0000375; P:RNA splicing, via transesterification reactions; IMP:UniProtKB. 
Interpro
 IPR007708; DBR1_C.
 IPR004843; Metallo_PEstase_dom. 
Pfam
 PF05011; DBR1
 PF00149; Metallophos 
SMART
  
PROSITE
  
PRINTS