CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014267
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 2 
Protein Synonyms/Alias
 Guanine nucleotide exchange factor H1; GEF-H1; LBC'S first cousin; Lymphoid blast crisis-like 1; Oncogene LFC; Rhobin 
Gene Name
 Arhgef2 
Gene Synonyms/Alias
 Kiaa0651; Lbcl1; Lfc 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
354TKALKLYKELYARDKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens (By similarity). 
Sequence Annotation
 DOMAIN 236 433 DH.
 DOMAIN 473 572 PH.
 ZN_FING 39 86 Phorbol-ester/DAG-type.
 MOD_RES 143 143 Phosphoserine; by PAK4 (By similarity).
 MOD_RES 163 163 Phosphoserine.
 MOD_RES 172 172 Phosphoserine (By similarity).
 MOD_RES 354 354 N6-acetyllysine (By similarity).
 MOD_RES 646 646 Phosphoserine.
 MOD_RES 649 649 Phosphoserine (By similarity).
 MOD_RES 680 680 Phosphothreonine; by MAPK1 or MAPK3 (By
 MOD_RES 692 692 Phosphoserine (By similarity).
 MOD_RES 781 781 Phosphoserine.
 MOD_RES 795 795 Phosphothreonine.
 MOD_RES 885 885 Phosphoserine; by PAK1 and AURKA (By
 MOD_RES 893 893 Phosphotyrosine (By similarity).
 MOD_RES 895 895 Phosphoserine; by PAK4 (By similarity).
 MOD_RES 931 931 Phosphoserine.
 MOD_RES 939 939 Phosphoserine.
 MOD_RES 940 940 Phosphoserine.
 MOD_RES 944 944 Phosphothreonine.
 MOD_RES 946 946 Phosphoserine (By similarity).
 MOD_RES 952 952 Phosphoserine (By similarity).
 MOD_RES 955 955 Phosphoserine.
 MOD_RES 959 959 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Cell cycle; Cell division; Cell junction; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Golgi apparatus; Guanine-nucleotide releasing factor; Metal-binding; Microtubule; Mitosis; Phosphoprotein; Proto-oncogene; Reference proteome; Tight junction; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 985 AA 
Protein Sequence
MSRIESLTRA RIDRSKEQAT KTREKEKMKE AKDARYTNGH LFTTISVSGM TMCYACNKSI 60
TAKEALICPT CNVTIHNRCK DTLANCTKVK QKQQKAALLR NNTALQSVSL RSKTTTRERP 120
TSAIYPSDSF RQSLLGSRRG LSSLSLAKSV STTNIAGHFN DESPLGLRQI LSQSTDSLNM 180
RNRTLSVESL IDEGVEVFYN ELMSDFEMDE KDFEADSWSL AVDSSFLQQH KKEVMKKQDV 240
IYELIQTELH HVRTLKIMTR LFRTGMLEEL QMEPEVVQGL FPCVDELSDI HTRFLNQLLE 300
RRRQALCPGS TRNFVIHRLG DLLISQFSGS NAEQMRKTYS EFCSRHTKAL KLYKELYARD 360
KRFQQFIRKM TRSAVLKRHG VQECILLVTQ RITKYPVLIN RILQNSHGVE EEYQDLASAL 420
GLVKELLSNV DQDVHELEKE ARLQEIYNRM DPRAQTPVPG KGPFGRDELL RRKLIHEGCL 480
LWKTATGRFK DVLLLLMTDV LVFLQEKDQK YIFTSLDKPS VVSLQNLIVR DIANQAKGMF 540
LISSGPPEMY EVHAASRDDR TTWIRVIQQS VRLCPSREDF PLIETEDKAY LRRIKTKLQQ 600
KNQALVELLQ KNVELFAEMV HFQALKAGFV GMPPPALPRG LFRLESFESL RGERLLKDAL 660
REVEGLKDLL LGPCVDLPMT SREPALPLDS DSGSCPGVTA NGEARTFNGS IELCRADSDS 720
SQKDRNGNQL RSPQEEVLQP LINLYGLLHG LQAVVVQQER LMEALFPEGP ERWEKLSRAN 780
SRDGEAGRAA VASVTPEKQA TELALLQRQH TLLQEELRRC QRLGEERATE AGSLEARLRE 840
SEQARALLER EAEEIRRQLA ALGQNEPLPA EAPWARRPLD PRRRSLPAGD ALYLSFNPPQ 900
PSRGHDRLDL PVTVRSLHRP FDDREAQELG SPEDRLQDSS DPDTGSEEEV SSRLSPPHSP 960
RDFTRMQDIP EETESRDGEP TASES 985 
Gene Ontology
 GO:0043198; C:dendritic shaft; IDA:MGI.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; ISS:UniProtKB.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0032587; C:ruffle membrane; ISS:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005923; C:tight junction; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; ISS:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0048365; F:Rac GTPase binding; ISS:UniProtKB.
 GO:0030676; F:Rac guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
 GO:0007015; P:actin filament organization; ISS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000902; P:cell morphogenesis; ISS:UniProtKB.
 GO:0000132; P:establishment of mitotic spindle orientation; IMP:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007026; P:negative regulation of microtubule depolymerization; ISS:UniProtKB.
 GO:0050768; P:negative regulation of neurogenesis; IMP:UniProtKB.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:0035023; P:regulation of Rho protein signal transduction; IDA:MGI. 
Interpro
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR015721; RhoGEF-like. 
Pfam
 PF00169; PH
 PF00621; RhoGEF 
SMART
 SM00109; C1
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS