UniProt Accession

 SNX4_HUMAN; O95219; B3KMH0; D3DNA3 

Genbank Protein ID

 AF065485; AK001835; CH471052; CH471052; CH471052; BC018762 

Genbank Nucleotide ID

 AAC83149.1; BAG50982.1; EAW79390.1; EAW79391.1; EAW79393.1; AAH18762.1 

Protein Name

 Sorting nexin-4 

Protein Synonyms/Alias

  

Gene Name

 SNX4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	GLGAAVGKEAEGAGE	ubiquitination	[1]
57	HNNFWLKKIEISVSE	ubiquitination	[1]
67	ISVSEAEKRTGRNAM	ubiquitination	[1]
132	VVPPLPEKRAEFVWH	ubiquitination	[1]
140	RAEFVWHKLSADNMD	ubiquitination	[1, 2, 3]
199	NETGFQLKADSRLKA	ubiquitination	[1, 2]
205	LKADSRLKALNATFR	ubiquitination	[1]
224	DKRFTDLKHYSDELQ	ubiquitination	[1, 2]
253	DRLYGVYKVHGNYGR	ubiquitination	[1, 4, 5]
320	ALRAVCRKHELMQYD	ubiquitination	[1]
339	AQDLASKKQQCEELV	ubiquitination	[1]
356	TVRTFSLKGMTTKLF	ubiquitination	[1]
361	SLKGMTTKLFGQETP	ubiquitination	[1, 2]
376	EQREARIKVLEEQIN	ubiquitination	[1, 2]
390	NEGEQQLKSKNLEGR	ubiquitination	[1, 2]
401	LEGREFVKNAWADIE	ubiquitination	[1, 2]
444	IQVWTNAKECFSKM*	ubiquitination	[1]
449	NAKECFSKM******	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 May be involved in several stages of intracellular trafficking. Plays a role in recycling endocytosed transferrin receptor and prevent its degradation. 

Sequence Annotation

 DOMAIN 61 187 PX.
 BINDING 106 106 Phosphatidylinositol 3-phosphate (By
 BINDING 108 108 Phosphatidylinositol 3-phosphate; via
 BINDING 132 132 Phosphatidylinositol 3-phosphate (By
 BINDING 154 154 Phosphatidylinositol 3-phosphate (By
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 22 22 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Endosome; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 450 AA 

Protein Sequence

Gene Ontology

 GO:0005868; C:cytoplasmic dynein complex; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IDA:UniProtKB.
 GO:0035091; F:phosphatidylinositol binding; IMP:UniProtKB.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0032456; P:endocytic recycling; IMP:UniProtKB.
 GO:0006897; P:endocytosis; TAS:ProtInc.
 GO:0015031; P:protein transport; IMP:UniProtKB. 

Interpro

 IPR001683; Phox. 

Pfam

 PF00787; PX 

SMART

 SM00312; PX 

PROSITE

 PS50195; PX 

PRINTS