UniProt Accession

 AUHM_MOUSE; Q9JLZ3; Q80YD7; Q8QZS0; Q9CY78; Q9D155 

Genbank Protein ID

 AF118386; AK003929; AK019978; BC026525; BC049597 

Genbank Nucleotide ID

 AAF28835.1; BAB23078.1; BAB31947.1; AAH26525.1; AAH49597.1 

Protein Name

 Methylglutaconyl-CoA hydratase, mitochondrial 

Protein Synonyms/Alias

 AU-specific RNA-binding enoyl-CoA hydratase; AU-binding enoyl-CoA hydratase; muAUH 

Gene Name

 Auh 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
75	GINRAYGKNALSKNL	acetylation	[1]
80	YGKNALSKNLLKMLS	acetylation	[1]
84	ALSKNLLKMLSKAVD	acetylation	[1]
88	NLLKMLSKAVDALKS	acetylation	[1]
94	SKAVDALKSDKKVRT	acetylation	[1]
97	VDALKSDKKVRTIII	acetylation	[1]
119	FCAGADLKERAKMHS	acetylation	[1]
179	RVAASSAKMGLVETK	acetylation	[1]
186	KMGLVETKLAIIPGG	acetylation	[1]
224	VLDGQEAKAVGLISH	acetylation	[1]
304	LEGLLAFKEKRPPRY	acetylation	[1, 2, 3]
304	LEGLLAFKEKRPPRY	succinylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Catalyzes the conversion of 3-methylglutaconyl-CoA to 3- hydroxy-3-methylglutaryl-CoA. Has very low enoyl-CoA hydratase activity. Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (By similarity). 

Sequence Annotation

 REGION 80 94 RNA-binding (By similarity).  

Keyword

 Alternative splicing; Branched-chain amino acid catabolism; Complete proteome; Lyase; Mitochondrion; Reference proteome; RNA-binding; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0004300; F:enoyl-CoA hydratase activity; ISS:UniProtKB.
 GO:0004490; F:methylglutaconyl-CoA hydratase activity; IEA:EC.
 GO:0003730; F:mRNA 3'-UTR binding; ISS:UniProtKB.
 GO:0006552; P:leucine catabolic process; IEA:UniProtKB-UniPathway.
 GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; TAS:MGI. 

Interpro

 IPR014748; Crontonase_C.
 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 

Pfam

 PF00378; ECH 

SMART

  

PROSITE

 PS00166; ENOYL_COA_HYDRATASE 

PRINTS