CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000280
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase RIO3 
Protein Synonyms/Alias
 RIO kinase 3; sudD homolog 
Gene Name
 RIOK3 
Gene Synonyms/Alias
 SUDD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
102QLRREEKKFNGDSKVubiquitination[1]
108KKFNGDSKVSISFENubiquitination[1, 2, 3]
146DDPYRPAKPVPTPKKubiquitination[4]
158PKKGFIGKGKDITTKubiquitination[3]
160KGFIGKGKDITTKHDubiquitination[3]
165KGKDITTKHDEVVCGubiquitination[3]
207NHVFNALKQHAYSEEubiquitination[2, 3]
290IPTECAIKVFKTTLNubiquitination[2]
293ECAIKVFKTTLNEFKubiquitination[3]
300KTTLNEFKNRDKYIKubiquitination[1, 2, 3, 4]
304NEFKNRDKYIKDDFRubiquitination[3]
307KNRDKYIKDDFRFKDubiquitination[1, 3]
318RFKDRFSKLNPRKIIubiquitination[2, 3]
452NVSQFFQKGGVKEALubiquitination[2, 3, 4]
456FFQKGGVKEALSEREubiquitination[2, 3, 4]
502HVQKNGRKAASFLKDubiquitination[3]
508RKAASFLKDDGDPPLubiquitination[3, 4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 DOMAIN 251 519 Protein kinase.
 NP_BIND 257 265 ATP (By similarity).
 ACT_SITE 406 406 Proton acceptor (By similarity).
 BINDING 290 290 ATP (By similarity).
 MOD_RES 122 122 Phosphotyrosine.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 128 128 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 519 AA 
Protein Sequence
MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA 60
VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH 120
PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME 180
NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL 240
MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK 300
NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS 360
FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW 420
LIDVSQSVEP THPHGLEFLF RDCRNVSQFF QKGGVKEALS ERELFNAVSG LNITADNEAD 480
FLAEIEALEK MNEDHVQKNG RKAASFLKDD GDPPLLYDE 519 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; TAS:ProtInc. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR018934; RIO-like_kinase.
 IPR000687; RIO_kinase.
 IPR018935; RIO_kinase_CS.
 IPR017406; Ser/Thr_kinase_Rio3. 
Pfam
 PF01163; RIO1 
SMART
 SM00090; RIO 
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS01245; RIO1 
PRINTS