CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002272
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 
Protein Synonyms/Alias
 Cobalamin-independent methionine synthase; Delta-P8 protein; Methionine synthase, vitamin-B12 independent isozyme 
Gene Name
 MET6 
Gene Synonyms/Alias
 YER091C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
37ITVDELFKVGKDLRTacetylation[1]
48DLRTQNWKLQKEAGVacetylation[1]
85VIPDRYTKYDLSPIDacetylation[1]
110RKATETEKAVDVTALacetylation[1]
121VTALEMVKWFDSNYHubiquitination[2]
141FSKTTQFKLNGQKPVacetylation[1]
146QFKLNGQKPVDEFLEacetylation[3]
146QFKLNGQKPVDEFLEubiquitination[2]
233NAQAAIKKAYTYFGEacetylation[1]
233NAQAAIKKAYTYFGEubiquitination[2]
307VDGRNIWKNDFKKSSacetylation[1]
319KSSAIVNKAIEKLGAacetylation[1]
323IVNKAIEKLGADRVVacetylation[1]
389AALEANAKSVESRGKacetylation[1]
398VESRGKSKFIHDAAVacetylation[1]
406FIHDAAVKARVASIDacetylation[1]
415RVASIDEKMSTRAAPacetylation[1]
415RVASIDEKMSTRAAPubiquitination[2]
432QRLPEQQKVFNLPLFubiquitination[2]
451IGSFPQTKDIRINRNacetylation[1]
451IGSFPQTKDIRINRNubiquitination[2]
462INRNKFNKGTISAEEacetylation[1]
472ISAEEYEKFINSEIEacetylation[1]
472ISAEEYEKFINSEIEubiquitination[2]
480FINSEIEKVIRFQEEacetylation[1]
559YAQSITSKPVKGMLTacetylation[1]
559YAQSITSKPVKGMLTubiquitination[2]
712HSPRIPSKDEFIAKIacetylation[1]
724AKISTILKSYPAEKFacetylation[1]
724AKISTILKSYPAEKFubiquitination[2]
730LKSYPAEKFWVNPDCubiquitination[2]
740VNPDCGLKTRGWEETacetylation[1]
740VNPDCGLKTRGWEETubiquitination[2]
759THMVEAAKYFREQYKacetylation[1]
759THMVEAAKYFREQYKubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 
Functional Description
 Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation. 
Sequence Annotation
 METAL 655 655 Zinc (By similarity).
 METAL 657 657 Zinc (By similarity).
 METAL 737 737 Zinc (By similarity).
 MOD_RES 89 89 Phosphoserine.
 MOD_RES 242 242 Phosphoserine.
 MOD_RES 566 566 Phosphothreonine.
 MOD_RES 629 629 Phosphoserine.
 MOD_RES 706 706 Phosphoserine.  
Keyword
 Amino-acid biosynthesis; Complete proteome; Direct protein sequencing; Metal-binding; Methionine biosynthesis; Methyltransferase; Phosphoprotein; Reference proteome; Transferase; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 767 AA 
Protein Sequence
MVQSAVLGFP RIGPNRELKK ATEGYWNGKI TVDELFKVGK DLRTQNWKLQ KEAGVDIIPS 60
NDFSFYDQVL DLSLLFNVIP DRYTKYDLSP IDTLFAMGRG LQRKATETEK AVDVTALEMV 120
KWFDSNYHYV RPTFSKTTQF KLNGQKPVDE FLEAKELGIH TRPVLLGPVS YLFLGKADKD 180
SLDLEPLSLL EQLLPLYTEI LSKLASAGAT EVQIDEPVLV LDLPANAQAA IKKAYTYFGE 240
QSNLPKITLA TYFGTVVPNL DAIKGLPVAA LHVDFVRAPE QFDEVVAAIG NKQTLSVGIV 300
DGRNIWKNDF KKSSAIVNKA IEKLGADRVV VATSSSLLHT PVDLNNETKL DAEIKGFFSF 360
ATQKLDEVVV ITKNVSGQDV AAALEANAKS VESRGKSKFI HDAAVKARVA SIDEKMSTRA 420
APFEQRLPEQ QKVFNLPLFP TTTIGSFPQT KDIRINRNKF NKGTISAEEY EKFINSEIEK 480
VIRFQEEIGL DVLVHGEPER NDMVQYFGEQ INGYAFTVNG WVQSYGSRYV RPPIIVGDLS 540
RPKAMSVKES VYAQSITSKP VKGMLTGPIT CLRWSFPRDD VDQKTQAMQL ALALRDEVND 600
LEAAGIKVIQ VDEPALREGL PLREGTERSA YYTWAAEAFR VATSGVANKT QIHSHFCYSD 660
LDPNHIKALD ADVVSIEFSK KDDANYIAEF KNYPNHIGLG LFDIHSPRIP SKDEFIAKIS 720
TILKSYPAEK FWVNPDCGLK TRGWEETRLS LTHMVEAAKY FREQYKN 767 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0003871; F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0009086; P:methionine biosynthetic process; IMP:SGD. 
Interpro
 IPR013215; Cbl-indep_Met_Synth_N.
 IPR006276; Cobalamin-indep_Met_synthase.
 IPR002629; Methionine_synth. 
Pfam
 PF08267; Meth_synt_1
 PF01717; Meth_synt_2 
SMART
  
PROSITE
  
PRINTS