CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016430
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 48 
Protein Synonyms/Alias
 USP1-associated factor 1 
Gene Name
 Wdr48 
Gene Synonyms/Alias
 Kiaa1449; Uaf1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
294RVLICEEKAPVLKMEubiquitination[1]
318AIWVATTKSTVNKWTubiquitination[1]
327TVNKWTLKGIHNFRAubiquitination[1]
400CKVEDLGKVDFEDEIubiquitination[1]
578PHASSGAKTLKKDRLacetylation[2]
621TSSSNNEKPEQEKEEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. May play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes (By similarity). 
Sequence Annotation
 REPEAT 28 67 WD 1.
 REPEAT 73 112 WD 2.
 REPEAT 115 154 WD 3.
 REPEAT 166 205 WD 4.
 REPEAT 208 247 WD 5.
 REPEAT 250 289 WD 6.
 REPEAT 292 334 WD 7.
 REPEAT 358 397 WD 8.
 MOD_RES 28 28 Phosphotyrosine.
 MOD_RES 214 214 N6-acetyllysine (By similarity).
 MOD_RES 338 338 Phosphotyrosine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Lysosome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 676 AA 
Protein Sequence
MAAHHRQNTA GRRKVQVSYV IRDEVEKYNR NGVNALQLDP ALNRLFTAGR DSIIRIWSVN 60
QHKQDPYIAS MEHHTDWVND VVLCCNGKTL ISASSDTTVK VWNAHKGFCM STLRTHKDYV 120
KALAYAKDKE LVASAGLDRQ IFLWDVNTLT ALTASNNTVT TSSLSGNKDS IYSLAMNQLG 180
TIIVSGSTEK VLRVWDPRTC AKLMKLKGHT DNVKALLLHR DGTQCLSGSS DGTIRLWSLG 240
QQRCIATYRV HDEGVWALQV NDAFTHVYSG GRDRKIYCTD LRNPDIRVLI CEEKAPVLKM 300
ELDRSADPPP AIWVATTKST VNKWTLKGIH NFRASGDYDN DCTNPITPLC TQPDQVIKGG 360
ASIIQCHILN DKRHILTKDT NNNVAYWDVL KACKVEDLGK VDFEDEIKKR FKMVYVPNWF 420
SVDLKTGMLT ITLDESDCFA AWVSAKDAGF SSPDGSDPKL NLGGLLLQAL LEYWPRTHVT 480
PMDEEENEVN HVSGGQESRV QKGNGYFQVP PHTPVIFGEA GGRTLFRLLC RDSGGETEAM 540
LLNETVPQWV IDITVDKNMP KFNKIPFYLQ PHASSGAKTL KKDRLSASDM LQVRKVMEHV 600
YEKIINLDNE SQTTSSSNNE KPEQEKEEDI AVLAEEKIEL LCQDQVLDPN MDLRTVKHFI 660
WKSGGDLTLH YRQKST 676 
Gene Ontology
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB. 
Interpro
 IPR021772; DUF3337.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF11816; DUF3337
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.