UniProt Accession

 YPK1_YEAST; P12688; D6VX69 

Genbank Protein ID

 M21307; Z28126; BK006944 

Genbank Nucleotide ID

 AAA34880.1; CAA81967.1; DAA09035.1 

Protein Name

 Serine/threonine-protein kinase YPK1 

Protein Synonyms/Alias

 Sphingosine-like immunosuppressant resistant protein 2; Yeast protein kinase 1 

Gene Name

 YPK1 

Gene Synonyms/Alias

 SLI2; YKL126W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
55	TRSSLDRKGTINPSN	ubiquitination	[1]
175	QLSRVQIKNQGPADE	ubiquitination	[1]
376	TQKVYALKAIRKSYI	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Plays an essential role in the proliferation of yeast cells. Involved in a signaling pathway, required for optimal cell wall integrity, that acts in parallel with the PKC1-SLT2-dependent pathway. Downstream kinase in the sphingolipid-mediated signaling pathway. Its phosphorylation is regulated by the intracellular sphingolipid concentration. Cooperates with SLI1 in mediating resistance to the sphingolipid biosynthesis inhibitor drug myriocin (ISP-1). Its kinase activity is essential for the resistance. Required for both receptor-mediated and fluid-phase endocytosis, but is not necessary for receptor phosphorylation or ubiquitination. Necessary for the internalization of plasma membrane proteins carrying different types of internalization signals. Acts downstream of the PKH kinases to control endocytosis by phosphorylating components of the endocytic machinery. Phosphorylation of residue Thr-504 in the activation loop is essential for activity. phosphorylates and down-regulates flippase activator FPK1. 

Sequence Annotation

 DOMAIN 347 602 Protein kinase.
 DOMAIN 603 673 AGC-kinase C-terminal.
 NP_BIND 353 361 ATP (By similarity).
 ACT_SITE 470 470 Proton acceptor (By similarity).
 BINDING 376 376 ATP (By similarity).
 MOD_RES 57 57 Phosphothreonine.
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 64 64 Phosphoserine.
 MOD_RES 71 71 Phosphoserine.
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 502 502 Phosphothreonine.
 MOD_RES 504 504 Phosphothreonine; by PKH1.
 MOD_RES 644 644 Phosphoserine.
 MOD_RES 653 653 Phosphoserine.
 MOD_RES 662 662 Phosphothreonine; by PKH1.
 MOD_RES 671 671 Phosphoserine.  

Keyword

 ATP-binding; Cell membrane; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Endocytosis; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 680 AA 

Protein Sequence

Gene Ontology

 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0070941; P:eisosome assembly; IGI:SGD.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0061093; P:negative regulation of phospholipid translocation; IMP:SGD.
 GO:0046777; P:protein autophosphorylation; IDA:SGD. 

Interpro

 IPR000961; AGC-kinase_C.
 IPR011009; Kinase-like_dom.
 IPR017892; Pkinase_C.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase
 PF00433; Pkinase_C 

SMART

 SM00133; S_TK_X
 SM00220; S_TKc 

PROSITE

 PS51285; AGC_KINASE_CTER
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS