CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002263
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor AP-2-alpha 
Protein Synonyms/Alias
 AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2 
Gene Name
 TFAP2A 
Gene Synonyms/Alias
 AP2TF; TFAP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10WKLTDNIKYEDCEDRsumoylation[1, 2]
315CETEFPAKAVAEFLNubiquitination[3]
Reference
 [1] Transcription factor AP-2 interacts with the SUMO-conjugating enzyme UBC9 and is sumolated in vivo.
 Eloranta JJ, Hurst HC.
 J Biol Chem. 2002 Aug 23;277(34):30798-804. [PMID: 12072434]
 [2] Alternative TFAP2A isoforms have distinct activities in breast cancer.
 Berlato C, Chan KV, Price AM, Canosa M, Scibetta AG, Hurst HC.
 Breast Cancer Res. 2011 Mar 4;13(2):R23. [PMID: 21375726]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. 
Sequence Annotation
 REGION 280 410 H-S-H (helix-span-helix), dimerization.
 MOTIF 57 62 WW-binding.
 MOD_RES 239 239 Phosphoserine; by PKA.
 CROSSLNK 10 10 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 437 AA 
Protein Sequence
MLWKLTDNIK YEDCEDRHDG TSNGTARLPQ LGTVGQSPYT SAPPLSHTPN ADFQPPYFPP 60
PYQPIYPQSQ DPYSHVNDPY SLNPLHAQPQ PQHPGWPGQR QSQESGLLHT HRGLPHQLSG 120
LDPRRDYRRH EDLLHGPHAL SSGLGDLSIH SLPHAIEEVP HVEDPGINIP DQTVIKKGPV 180
SLSKSNSNAV SAIPINKDNL FGGVVNPNEV FCSVPGRLSL LSSTSKYKVT VAEVQRRLSP 240
PECLNASLLG GVLRRAKSKN GGRSLREKLD KIGLNLPAGR RKAANVTLLT SLVEGEAVHL 300
ARDFGYVCET EFPAKAVAEF LNRQHSDPNE QVTRKNMLLA TKQICKEFTD LLAQDRSPLG 360
NSRPNPILEP GIQSCLTHFN LISHGFGSPA VCAAVTALQN YLTEALKAMD KMYLSNNPNS 420
HTDNNAKSSD KEEKHRK 437 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; TAS:UniProtKB.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:UniProtKB.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:UniProtKB.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:UniProtKB.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:UniProtKB.
 GO:0000980; F:RNA polymerase II distal enhancer sequence-specific DNA binding; IEA:Compara.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; IEA:Compara.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0021506; P:anterior neuropore closure; IEA:Compara.
 GO:0071711; P:basement membrane organization; IEA:Compara.
 GO:0060349; P:bone morphogenesis; ISS:UniProtKB.
 GO:0071281; P:cellular response to iron ion; IDA:UniProtKB.
 GO:0061303; P:cornea development in camera-type eye; IEA:Compara.
 GO:0010172; P:embryonic body morphogenesis; IEA:Compara.
 GO:0048701; P:embryonic cranial skeleton morphogenesis; ISS:UniProtKB.
 GO:0035115; P:embryonic forelimb morphogenesis; ISS:UniProtKB.
 GO:0009880; P:embryonic pattern specification; IEA:Compara.
 GO:0048730; P:epidermis morphogenesis; IEA:Compara.
 GO:0061029; P:eyelid development in camera-type eye; ISS:UniProtKB.
 GO:0060325; P:face morphogenesis; IEA:Compara.
 GO:0021884; P:forebrain neuron development; IEA:Compara.
 GO:0042472; P:inner ear morphogenesis; IMP:UniProtKB.
 GO:0003334; P:keratinocyte development; IEA:Compara.
 GO:0001822; P:kidney development; IMP:UniProtKB.
 GO:0060235; P:lens induction in camera-type eye; IEA:Compara.
 GO:0072210; P:metanephric nephron development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; IEA:Compara.
 GO:2000378; P:negative regulation of reactive oxygen species metabolic process; IDA:UniProtKB.
 GO:0010944; P:negative regulation of transcription by competitive promoter binding; IDA:UniProtKB.
 GO:0014032; P:neural crest cell development; IEA:Compara.
 GO:0021623; P:oculomotor nerve formation; ISS:UniProtKB.
 GO:0003409; P:optic cup structural organization; ISS:UniProtKB.
 GO:0003404; P:optic vesicle morphogenesis; ISS:UniProtKB.
 GO:0003151; P:outflow tract morphogenesis; IEA:Compara.
 GO:0060021; P:palate development; IMP:UniProtKB.
 GO:0030501; P:positive regulation of bone mineralization; IDA:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0043525; P:positive regulation of neuron apoptotic process; IDA:UniProtKB.
 GO:0070172; P:positive regulation of tooth mineralization; IDA:UniProtKB.
 GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
 GO:0045664; P:regulation of neuron differentiation; IEA:Compara.
 GO:0010842; P:retina layer formation; IEP:UniProtKB.
 GO:0007605; P:sensory perception of sound; IMP:UniProtKB.
 GO:0043588; P:skin development; IEA:Compara.
 GO:0048485; P:sympathetic nervous system development; IEA:Compara.
 GO:0021559; P:trigeminal nerve development; ISS:UniProtKB. 
Interpro
 IPR004979; TF_AP2.
 IPR008121; TF_AP2_alpha_N.
 IPR013854; TF_AP2_C. 
Pfam
 PF03299; TF_AP-2 
SMART
  
PROSITE
  
PRINTS
 PR01749; AP2ATNSCPFCT.
 PR01748; AP2TNSCPFCT.