CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001387
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bromodomain-containing protein 7 
Protein Synonyms/Alias
 75 kDa bromodomain protein 
Gene Name
 Brd7 
Gene Synonyms/Alias
 Bp75 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
197YQSIEELKDNFKLMCubiquitination[1]
328VVQESGGKLTRRLANacetylation[2, 3]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase (By similarity). Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24- mediated activation of transcriptional activation by AR. 
Sequence Annotation
 DOMAIN 148 218 Bromo.
 MOTIF 65 96 Nuclear localization signal (By
 MOD_RES 279 279 Phosphoserine.  
Keyword
 Bromodomain; Cell cycle; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Tumor suppressor; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 651 AA 
Protein Sequence
MGKKHKKHKS DRHFYEEYVE KPLKLVLKVG GSEVTELSTG SSGHDSSLFE DRSDHDKHKD 60
RKRKKRKKGE KQAPGEEKGR KRRRVKEDKK KRDRDRAENE VDRDLQCHVP IRLDLPPEKP 120
LTSSLAKQEE VEQTPLQEAL NQLMRQLQRK DPSAFFSFPV TDFIAPGYSM IIKHPMDFST 180
MKEKIKNNDY QSIEELKDNF KLMCTNAMIY NKPETIYYKA AKKLLHSGMK ILSQERIQSL 240
KQSIDFMSDL QKTRKQKERT DACQSGEDSG CWQREREDSG DAETQAFRSP AKDNKRKDKD 300
VLEDKWRSSN SEREHEQIER VVQESGGKLT RRLANSQCEF ERRKPDGTTT LGLLHPVDPI 360
VGEPGYCPVR LGMTTGRLQS GVNTLQGFKE DKRNRVTPVL YLNYGPYSSY APHYDSTFAN 420
ISKDDSDLIY STYGEDSDLP NNFSISEFLA TCQDYPYVMA DSLLDVLTKG GHSRSLQDLD 480
MSSPEDEGQT RALDTAKEAE ITQIEPTGRL ESSSQDRLTA LQAVTTFGAP AEVFDSEEAE 540
VFQRKLDETT RLLRELQEAQ NERLSTRPPP NMICLLGPSY REMYLAEQVT NNLKELTQQV 600
TPGDVVSIHG VRKAMGISVP SPIVGNSFVD LTGECEEPKE TSTAECGPDA S 651 
Gene Ontology
 GO:0005634; C:nucleus; IPI:MGI.
 GO:0070577; F:histone acetyl-lysine binding; ISS:UniProtKB.
 GO:0002039; F:p53 binding; ISS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0008134; F:transcription factor binding; ISA:MGI.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:Compara.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
 GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; ISS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0035066; P:positive regulation of histone acetylation; ISS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR001487; Bromodomain.
 IPR021900; DUF3512. 
Pfam
 PF00439; Bromodomain
 PF12024; DUF3512 
SMART
 SM00297; BROMO 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2 
PRINTS
 PR00503; BROMODOMAIN.