CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000402
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prefoldin subunit 6 
Protein Synonyms/Alias
 Protein Ke2 
Gene Name
 PFDN6 
Gene Synonyms/Alias
 HKE2; PFD6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MAELIQKKLQGEVEubiquitination[1]
8MAELIQKKLQGEVEKubiquitination[1]
15KLQGEVEKYQQLQKDubiquitination[1, 2, 3]
21EKYQQLQKDLSKSMSacetylation[4]
21EKYQQLQKDLSKSMSubiquitination[1]
25QLQKDLSKSMSGRQKubiquitination[1]
66LLGPVLVKQELGEARacetylation[4]
66LLGPVLVKQELGEARubiquitination[1, 2, 3, 5, 6]
88DYITAEIKRYESQLRubiquitination[1, 2, 3, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. 
Sequence Annotation
 MOD_RES 21 21 N6-acetyllysine.
 MOD_RES 66 66 N6-acetyllysine.  
Keyword
 Acetylation; Chaperone; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 129 AA 
Protein Sequence
MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL 60
GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA 120
AKAGAPGKA 129 
Gene Ontology
 GO:0016272; C:prefoldin complex; IDA:UniProtKB.
 GO:0051087; F:chaperone binding; IDA:UniProtKB.
 GO:0051082; F:unfolded protein binding; NAS:UniProtKB.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome.
 GO:0051131; P:chaperone-mediated protein complex assembly; IDA:UniProtKB. 
Interpro
 IPR002777; PFD_beta-like.
 IPR009053; Prefoldin. 
Pfam
 PF01920; Prefoldin_2 
SMART
  
PROSITE
  
PRINTS