CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020172
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fibronectin type III and SPRY domain-containing protein 1 
Protein Synonyms/Alias
 MID1-related protein 1; Microtubule-associated protein GLFND 
Gene Name
 FSD1 
Gene Synonyms/Alias
 GLFND; MIR1; VLP27 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13EALRKIIKTLAVKNEubiquitination[1, 2, 3]
214FEGPPRLKEDQPWMVubiquitination[2]
240TGLKFDMKYMNFRVKubiquitination[2]
367VRYEPDSKAFGVGVAubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May be involved in microtubule organization and stabilization. 
Sequence Annotation
 DOMAIN 105 162 COS.
 DOMAIN 165 267 Fibronectin type-III.
 DOMAIN 268 477 B30.2/SPRY.  
Keyword
 Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 496 AA 
Protein Sequence
MEEQREALRK IIKTLAVKNE EIQSFIYSLK QMLLNVEANS AKVQEDLEAE FQSLFSLLEE 60
LKEGMLMKIK QDRASRTYEL QNQLAACTRA LESSEELLET ANQTLQAMDS EDFPQAAKQI 120
KDGVTMAPAF RLSLKAKVSD NMSHLMVDFA QERQMLQALK FLPVPSAPVI DLAESLVADN 180
CVTLVWRMPD EDSKIDHYVL EYRRTNFEGP PRLKEDQPWM VIEGIRQTEY TLTGLKFDMK 240
YMNFRVKACN KAVAGEFSEP VTLETPAFMF RLDASTSHQN LRVDDLSVEW DAMGGKVQDI 300
KAREKDGKGR TASPINSPAR GTPSPKRMPS GRGGRDRFTA ESYTVLGDTL IDGGEHYWEV 360
RYEPDSKAFG VGVAYRSLGR FEQLGKTAAS WCLHVNNWLQ VSFTAKHANK VKVLDAPVPD 420
CLGVHCDFHQ GLLSFYNART KQVLHTFKTR FTQPLLPAFT VWCGSFQVTT GLQVPSAVRC 480
LQKRGSATSS SNTSLT 496 
Gene Ontology
 GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR003649; Bbox_C.
 IPR008985; ConA-like_lec_gl_sf.
 IPR017903; COS_domain.
 IPR003961; Fibronectin_type3.
 IPR013783; Ig-like_fold.
 IPR003877; SPRY_rcpt. 
Pfam
 PF00041; fn3
 PF00622; SPRY 
SMART
 SM00502; BBC
 SM00060; FN3 
PROSITE
 PS50188; B302_SPRY
 PS51262; COS
 PS50853; FN3 
PRINTS