CPLM-000323

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-000323

UniProt Accession

RGS12_HUMAN; O14924; B1AQ30; B1AQ31; B1AQ32; O14922; O14923; O43510; O75338

Genbank Protein ID

AF030112; AF030111; AF030110; AF030109; AF030149; AF030151; AF030152; AF030135; AF030136; AF030137; AF030138; AF030139; AF030140; AF030141; AF030142; AF030143; AF030144; AF030145; AF030146; AF030147; AF030148; AF030149; AF030135; AF030136; AF030137; AF030138; AF030139; AF030140; AF030141; AF030142; AF030143; AF030144; AF030145; AF030146; AF030147; AF030148; AF030149; AF030134; AF030135; AF030136; AF030137; AF030138; AF030139; AF030140; AF030141; AF030142; AF030143; AF030144; AF030145; AF030146; AF030147; AF030148; AF035152; AL645949; AL590235; AL645949; AL590235; AL645949; AL590235; AL590235; AL645949; AL590235; AL645949; AL590235; AL645949; CH471131; CH471131; CH471131

Genbank Nucleotide ID

AAB84187.1; AAB84114.1; AAB84007.1; AAB84186.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96645.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96646.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAB96644.1; AAC39835.1; CAM17750.1; CAM17750.1; CAM17751.1; CAM17751.1; CAM17752.1; CAM17752.1; CAM21452.1; CAM21452.1; CAM21453.1; CAM21453.1; CAM21454.1; CAM21454.1; EAW82468.1; EAW82469.1; EAW82470.1

Protein Name

Regulator of G-protein signaling 12

Protein Synonyms/Alias

RGS12

Gene Name

RGS12

Gene Synonyms/Alias

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
1115	RGKASADKQKGVPVK	ubiquitination	[1, 2]

Reference

[1] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.

Sequence Annotation

DOMAIN 22 98 PDZ.
DOMAIN 228 340 PID.
DOMAIN 715 832 RGS.
DOMAIN 962 1032 RBD 1.
DOMAIN 1034 1104 RBD 2.
DOMAIN 1187 1209 GoLoco.
CROSSLNK 1115 1115 Glycyl lysine isopeptide (Lys-Gly)

Keyword

3D-structure; Alternative splicing; Complete proteome; Isopeptide bond; Nucleus; Polymorphism; Reference proteome; Repeat; Signal transduction inhibitor; Ubl conjugation.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1447 AA

Protein Sequence

MFRAGEASKR PLPGPSPPRV RSVEVARGRA GYGFTLSGQA PCVLSCVMRG SPADFVGLRA 60
GDQILAVNEI NVKKASHEDV VKLIGKCSGV LHMVIAEGVG RFESCSSDEE GGLYEGKGWL 120
KPKLDSKALG INRAERVVEE MQSGGIFNMI FENPSLCASN SEPLKLKQRS LSESAATRFD 180
VGHESINNPN PNMLSKEEIS KVIHDDSVFS IGLESHDDFA LDASILNVAM IVGYLGSIEL 240
PSTSSNLESD SLQAIRGCMR RLRAEQKIHS LVTMKIMHDC VQLSTDKAGV VAEYPAEKLA 300
FSAVCPDDRR FFGLVTMQTN DDGSLAQEEE GALRTSCHVF MVDPDLFNHK IHQGIARRFG 360
FECTADPDTN GCLEFPASSL PVLQFISVLY RDMGELIEGM RARAFLDGDA DAHQNNSTSS 420
NSDSGIGNFH QEEKSNRVLV VDLGGSSSRH GPGGSAWDGV GGRGAQPWGA PWTGPFCPDP 480
EGSPPFEAAH QTDRFWDLNK HLGPASPVEV PPASLRSSVP PSKRGTVGAG CGFNQRWLPV 540
HVLREWQCGH TSDQDSYTDS TDGWSSINCG TLPPPMSKIP ADRYRVEGSF AQPPLNAPKR 600
EWSRKAFGMQ SIFGPHRNVR KTKEDKKGSK FGRGTGLTQP SQRTSARRSF GRSKRFSITR 660
SLDDLESATV SDGELTGADL KDCVSNNSLS SNASLPSVQS CRRLRERRVA SWAVSFERLL 720
QDPVGVRYFS DFLRKEFSEE NILFWQACEY FNHVPAHDKK ELSYRAREIF SKFLCSKATT 780
PVNIDSQAQL ADDVLRAPHP DMFKEQQLQI FNLMKFDSYT RFLKSPLYQE CILAEVEGRA 840
LPDSQQVPSS PASKHSLGSD HSSVSTPKKL SGKSKSGRSL NEELGDEDSE KKRKGAFFSW 900
SRTRSTGRSQ KKREHGDHAD DALHANGGLC RRESQGSVSS AGSLDLSEAC RTLAPEKDKA 960
TKHCCIHLPD GTSCVVAVKA GFSIKDILSG LCERHGINGA AADLFLVGGD KPLVLHQDSS 1020
ILESRDLRLE KRTLFRLDLV PINRSVGLKA KPTKPVTEVL RPVVARYGLD LSGLLVRLSG 1080
EKEPLDLGAP ISSLDGQRVV LEEKDPSRGK ASADKQKGVP VKQNTAVNSS SRNHSATGEE 1140
RTLGKSNSIK IKGENGKNAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN 1200
RADDQRGLLR KEDLVLPEFL RLPPGSTELT LPTPAAVAKG FSKRSATGNG RESASQPGEQ 1260
WEPVQESSDS PSTSPGSASS PPGPPGTTPP GQKSPSGPFC TPQSPVSLAQ EGTAQIWKRQ 1320
SQEVEAGGIQ TVEDEHVAEL TLMGEGDISS PNSTLLPPPS TPQEVPGPSR PGSGTHGSRD 1380
LPVNRIIDVD LVTGSAPGRD GGIAGAQAGP GRSQASGGPP TSDLPGLGPV PGEPAKPKTS 1440
AHHATFV 1447

Gene Ontology

GO:0000794; C:condensed nuclear chromosome; TAS:ProtInc.
GO:0005737; C:cytoplasm; IBA:RefGenome.
GO:0005886; C:plasma membrane; IBA:RefGenome.
GO:0005096; F:GTPase activator activity; IBA:RefGenome.
GO:0005057; F:receptor signaling protein activity; IEA:InterPro.
GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; TAS:ProtInc.
GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro.

Interpro

IPR003109; GoLoco_motif.
IPR001478; PDZ.
IPR011993; PH_like_dom.
IPR006020; PTyr_interaction_dom.
IPR003116; Raf-like_ras-bd.
IPR000342; Regulat_G_prot_signal.
IPR024066; Regulat_G_prot_signal_dom1.
IPR016137; Regulat_G_prot_signal_superfam.

Pfam

PF02188; GoLoco
PF00595; PDZ
PF02196; RBD
PF00615; RGS

SMART

SM00390; GoLoco
SM00228; PDZ
SM00462; PTB
SM00455; RBD
SM00315; RGS

PROSITE

PS50877; GOLOCO
PS50106; PDZ
PS01179; PID
PS50898; RBD
PS50132; RGS

PRINTS

PR01301; RGSPROTEIN.