| Tag | Content |
|---|
| CPLM ID | CPLM-001717 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 |
Protein Synonyms/Alias | 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ARF GTPase-activating protein 1; Development and differentiation-enhancing factor 1; DEF-1; Differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP |
Gene Name | ASAP1 |
Gene Synonyms/Alias | DDEF1 |
Created Date | July 27, 2013 |
Organism | Bos taurus (Bovine) |
NCBI Taxa ID | 9913 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 232 | VNEIKTKKGVDLLQN | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Possesses phosphatidylinositol 4,5-bisphosphate- dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. Plays a role in ciliogenesis (By similarity). May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. |
Sequence Annotation | DOMAIN 327 419 PH.
DOMAIN 442 565 Arf-GAP.
REPEAT 603 635 ANK 1.
REPEAT 639 668 ANK 2.
DOMAIN 1067 1129 SH3.
ZN_FING 457 480 C4-type.
MOD_RES 720 720 Phosphoserine (By similarity).
MOD_RES 846 846 Phosphoserine (By similarity).
MOD_RES 1014 1014 Phosphoserine (By similarity).
MOD_RES 1033 1033 Phosphoserine (By similarity). |
Keyword | ANK repeat; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Direct protein sequencing; GTPase activation; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; SH3 domain; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1129 AA |
Protein Sequence | MRSSASRLSS FSSRDSLWNR MPDQISVSEF IAETTEDYNS PTTSSFTTRL HNCRNTVTLL 60
EEALDQDRTA LQKVKKSVKA IYNSGQDHVQ NEENYAQVLD KFGSNFLSRD NPDLGTAFVK 120
FSTLTKELST LLKNLLQGLS HNVIFTLDSL LKGDLKGVKG DLKKPFDKAW KDYETKFTKI 180
EKEKREHAKQ HGMIRTEITG AEIAEEMEKE RRLFQLQMCE YLIKVNEIKT KKGVDLLQNL 240
IKYYHAQCNF FQDGLKTADK LKQYIEKLAA DLYNIKQTQD EEKKQLTALR DLIKSSLQLD 300
QKESRRDSQS RQGGYSMHQL QGNKEYGSEK KGYLLKKSDG IRKVWQRRKC SVKNGILTIS 360
HATSNRQPAK LNLLTCQVKP NAEDKKSFDL ISHNRTYHFQ AEDEQDYVAW ISVLTNSKEE 420
ALTMAFRGEQ SAGESSLEEL TKAIIEDVQR LPGNDVCCDC GSAEPTWLST NLGILTCIEC 480
SGIHREMGVH ISRIQSLELD KLGTSELLLA KNVGNNSFND IMEANLPSPS PKPTPSSDMT 540
VRKEYITAKY VDHRFSRKTC SSSSAKLNEL LEAIKSRDLL ALIQVYAEGV ELMEPLLEPG 600
QELGETALHL AVRTADQTSL HLVDFLVQNC GNLDKQTALG NTALHYCSMY SKPECLKLLL 660
RSKPTVDVVN QAGETALDIA KRLKATQCED LLSQAKSGKF NPHVHVEYEW NLRQEEMDES 720
DDDLDDKPSP IKKERSPRPQ SFCHSSSISP QDKLSLPGFS TPRDKQRLSY GAFTNQIFVS 780
TSTDSPTSPI AEAPPLPPRN ATKGPPGPPS TLPLSTQTSS GSSTLSKKRS PPPPPGHKRT 840
LSDPPSPLPH GPPNKGAVPW GNDVGPSSSS KTTNKFEGLS QQSSTGSAKT ALGPRVLPKL 900
PQKVALRKTE TSHHLSLDKA NVPPEIFQKS SQLTELPQKP PPGDLPPKPT ELAPKPPIGD 960
LPPKPGELPP KPQLGDLPPK PQLADLPPKP QVKDLPPKPQ LGELLAKPQT GDASPKAQPP 1020
LELTPKSHPA DLSPNVPKQA SEDTNDLTPT LPETPVPLPR KINTGKSKVR RVKTIYDCQA 1080
DNDDELTFME GEVIVVTGEE DQEWWIGHIE GQPERKGVFP VSFVHILSD 1129 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0016020; C:membrane; IEA:UniProtKB-SubCell. GO:0008060; F:ARF GTPase activator activity; IEA:InterPro. GO:0005543; F:phospholipid binding; IEA:InterPro. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0060271; P:cilium morphogenesis; ISS:UniProtKB. GO:0043547; P:positive regulation of GTPase activity; IEA:GOC. GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |