CPLM-019261

Genbank Nucleotide ID

OTU domain-containing protein 5

Protein Synonyms/Alias

Deubiquitinating enzyme A; DUBA

Homo sapiens (Human)

Position	Peptide	Type	References
205	QQEHWFEKALRDKKG	ubiquitination	[1, 2, 3, 4, 5]
219	GFIIKQMKEDGACLF	ubiquitination	[4, 6]
245	DMHEVVRKHCMDYLM	ubiquitination	[4]
343	NSVVNPNKATIGVGL	ubiquitination	[1, 5]
356	GLGLPSFKPGFAEQS	ubiquitination	[1, 2, 4, 5, 6, 7]
370	SLMKNAIKTSEESWI	ubiquitination	[2, 4]
385	EQQMLEDKKRATDWE	ubiquitination	[2, 3, 4, 5, 8]
386	QQMLEDKKRATDWEA	ubiquitination	[3, 4]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]

Functional Description

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'- linked ubiquitin chains (in vitro).

DOMAIN 213 341 OTU.
ACT_SITE 221 221 Potential.
ACT_SITE 224 224 Nucleophile.
ACT_SITE 334 334 Probable.
MOD_RES 64 64 Phosphoserine.
MOD_RES 165 165 Phosphoserine.
MOD_RES 175 175 Phosphotyrosine.
MOD_RES 177 177 Phosphoserine.
MOD_RES 452 452 Phosphoserine.
MOD_RES 507 507 Phosphothreonine.
MOD_RES 508 508 Phosphoserine (By similarity).

3D-structure; Alternative splicing; Complete proteome; Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0032480; P:negative regulation of type I interferon production; TAS:Reactome.
GO:0071108; P:protein K48-linked deubiquitination; IDA:UniProtKB.
GO:0070536; P:protein K63-linked deubiquitination; IDA:UniProtKB.
GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
GO:0032496; P:response to lipopolysaccharide; IDA:UniProtKB.