UniProt Accession

 ACY1_HUMAN; Q03154; C9J6I6; C9J9D8; C9JWD4 

Genbank Protein ID

 L07548; D14524; D16307; AC115284; BC000545; BC003023; BC014112 

Genbank Nucleotide ID

 AAA02852.1; BAA03397.1; BAA03814.1; AAH00545.1; AAH03023.1; AAH14112.1 

Protein Name

 Aminoacylase-1 

Protein Synonyms/Alias

 ACY-1; N-acyl-L-amino-acid amidohydrolase 

Gene Name

 ACY1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MTSKGPEEEHP	ubiquitination	[1]
28	RIRTVQPKPDYGAAV	ubiquitination	[1]
100	HDPFEAFKDSEGYIY	ubiquitination	[1]
115	ARGAQDMKCVSIQYL	ubiquitination	[1]
218	MEDTAAEKLHKVVNS	ubiquitination	[1]
221	TAAEKLHKVVNSILA	ubiquitination	[1]
244	LQSNPHLKEGSVTSV	ubiquitination	[1, 2]
332	AAFSRVCKDMNLTLE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). 

Sequence Annotation

 ACT_SITE 82 82 By similarity.
 ACT_SITE 147 147 Proton acceptor.
 METAL 80 80 Zinc 1.
 METAL 113 113 Zinc 1.
 METAL 113 113 Zinc 2.
 METAL 148 148 Zinc 2.
 METAL 175 175 Zinc 1.
 METAL 373 373 Zinc 2.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Hydrolase; Metal-binding; Polymorphism; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 408 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:ProtInc.
 GO:0004046; F:aminoacylase activity; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IEA:InterPro.
 GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:InterPro. 

Interpro

 IPR001261; ArgE/DapE_CS.
 IPR010159; N-acyl_aa_amidohydrolase.
 IPR002933; Peptidase_M20.
 IPR011650; Peptidase_M20_dimer. 

Pfam

 PF07687; M20_dimer
 PF01546; Peptidase_M20 

SMART

  

PROSITE

 PS00758; ARGE_DAPE_CPG2_1
 PS00759; ARGE_DAPE_CPG2_2 

PRINTS