UniProt Accession

 TIM50_HUMAN; Q3ZCQ8; Q6QA00; Q96FJ5; Q96GY2; Q9H370 

Genbank Protein ID

 AY551341; BC009072; BC010736; BC050082; AF130109 

Genbank Nucleotide ID

 AAT01208.1; AAH09072.1; AAH10736.1; AAH50082.1; AAG35534.1 

Protein Name

 Mitochondrial import inner membrane translocase subunit TIM50 

Protein Synonyms/Alias

  

Gene Name

 TIMM50 

Gene Synonyms/Alias

 TIM50; PRO1512 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
150	IGSRGSTKAQGPQQQ	ubiquitination	[1]
167	SEGPSYAKKVALWLA	ubiquitination	[1]
223	RRTYKYFKDYRQMII	ubiquitination	[2]
335	YMDGHHVKDISCLNR	ubiquitination	[2, 3]
354	VVVVDCKKEAFRLQP	ubiquitination	[2]
415	DDPLAAFKQRQSRLE	ubiquitination	[1, 2, 3]
434	QRLAELSKSNKQNLF	ubiquitination	[2]
437	AELSKSNKQNLFLGS	ubiquitination	[1, 2, 3]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. 

Sequence Annotation

 DOMAIN 143 286 FCP1 homology.  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleus; Protein transport; Reference proteome; RNA-binding; Transit peptide; Translocation; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 353 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005744; C:mitochondrial inner membrane presequence translocase complex; IPI:UniProtKB.
 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0005134; F:interleukin-2 receptor binding; IDA:MGI.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; IDA:UniProtKB.
 GO:0043021; F:ribonucleoprotein complex binding; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007006; P:mitochondrial membrane organization; IMP:UniProtKB.
 GO:0006626; P:protein targeting to mitochondrion; TAS:Reactome.
 GO:0001836; P:release of cytochrome c from mitochondria; IDA:MGI. 

Interpro

 IPR023214; HAD-like_dom.
 IPR004274; NIF.
 IPR027111; Tim50. 

Pfam

 PF03031; NIF 

SMART

 SM00577; CPDc 

PROSITE

 PS50969; FCP1 

PRINTS