CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009526
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform 
Protein Synonyms/Alias
 PP2A-beta 
Gene Name
 PPP2CB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MDDKAFTKELDubiquitination[1]
8MDDKAFTKELDQWVEubiquitination[1]
21VEQLNECKQLNENQVubiquitination[1, 2, 3, 4, 5]
36RTLCEKAKEILTKESubiquitination[3]
41KAKEILTKESNVQEVubiquitination[1, 3, 5, 6]
74ELFRIGGKSPDTNYLubiquitination[1, 5]
136FYDECLRKYGNANVWubiquitination[1]
283MELDDTLKYSFLQFDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. 
Sequence Annotation
 ACT_SITE 118 118 Proton donor (By similarity).
 METAL 57 57 Iron (By similarity).
 METAL 59 59 Iron (By similarity).
 METAL 85 85 Iron (By similarity).
 METAL 85 85 Manganese (By similarity).
 METAL 117 117 Manganese (By similarity).
 METAL 167 167 Manganese (By similarity).
 METAL 241 241 Manganese (By similarity).
 MOD_RES 307 307 Phosphotyrosine (By similarity).
 MOD_RES 309 309 Leucine methyl ester.  
Keyword
 Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Hydrolase; Iron; Manganese; Metal-binding; Methylation; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MDDKAFTKEL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC PVTVCGDVHG 60
QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYPER ITILRGNHES 120
RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI 180
RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA 240
HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
TRRTPDYFL 309 
Gene Ontology
 GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000159; C:protein phosphatase type 2A complex; TAS:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004722; F:protein serine/threonine phosphatase activity; IEA:Compara.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0006470; P:protein dephosphorylation; TAS:UniProtKB.
 GO:0010468; P:regulation of gene expression; IEA:Compara.
 GO:0046677; P:response to antibiotic; IEA:Compara.
 GO:0042542; P:response to hydrogen peroxide; IEA:Compara. 
Interpro
 IPR004843; Metallo_PEstase_dom.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 
Pfam
 PF00149; Metallophos 
SMART
 SM00156; PP2Ac 
PROSITE
 PS00125; SER_THR_PHOSPHATASE 
PRINTS
 PR00114; STPHPHTASE.