CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004748
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prothrombin 
Protein Synonyms/Alias
 Coagulation factor II; Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain 
Gene Name
 F2 
Gene Synonyms/Alias
 Cf2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
258QDFDPEVKLVENFCRubiquitination[1]
341GLRPLFEKKSLKDTTubiquitination[1]
350SLKDTTEKELLDSYIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity). 
Sequence Annotation
 DOMAIN 44 90 Gla.
 DOMAIN 109 187 Kringle 1.
 DOMAIN 215 292 Kringle 2.
 DOMAIN 361 615 Peptidase S1.
 REGION 548 570 High affinity receptor-binding region
 ACT_SITE 403 403 Charge relay system (By similarity).
 ACT_SITE 459 459 Charge relay system (By similarity).
 ACT_SITE 565 565 Charge relay system (By similarity).
 MOD_RES 50 50 4-carboxyglutamate.
 MOD_RES 51 51 4-carboxyglutamate.
 MOD_RES 58 58 4-carboxyglutamate.
 MOD_RES 60 60 4-carboxyglutamate.
 MOD_RES 63 63 4-carboxyglutamate.
 MOD_RES 64 64 4-carboxyglutamate.
 MOD_RES 69 69 4-carboxyglutamate.
 MOD_RES 70 70 4-carboxyglutamate.
 MOD_RES 73 73 4-carboxyglutamate.
 MOD_RES 76 76 4-carboxyglutamate.
 CARBOHYD 122 122 N-linked (GlcNAc...).
 CARBOHYD 144 144 N-linked (GlcNAc...).
 CARBOHYD 413 413 N-linked (GlcNAc...).
 CARBOHYD 553 553 N-linked (GlcNAc...).
 DISULFID 61 66 By similarity.
 DISULFID 91 104 By similarity.
 DISULFID 109 187 By similarity.
 DISULFID 130 170 By similarity.
 DISULFID 158 182 By similarity.
 DISULFID 215 293 By similarity.
 DISULFID 236 276 By similarity.
 DISULFID 264 288 By similarity.
 DISULFID 333 479 Interchain (between light and heavy
 DISULFID 388 404
 DISULFID 533 547
 DISULFID 561 591  
Keyword
 3D-structure; Acute phase; Blood coagulation; Calcium; Cleavage on pair of basic residues; Complete proteome; Disulfide bond; Gamma-carboxyglutamic acid; Glycoprotein; Hemostasis; Hydrolase; Kringle; Protease; Reference proteome; Repeat; Serine protease; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 618 AA 
Protein Sequence
MSHVRGLGLP GCLALAALVS LVHSQHVFLA PQQALSLLQR VRRANSGFLE ELRKGNLERE 60
CVEEQCSYEE AFEALESPQD TDVFWAKYTV CDSVRKPRET FMDCLEGRCA MDLGVNYLGT 120
VNVTHTGIQC QLWRSRYPHK PEINSTTHPG ADLKENFCRN PDSSTTGPWC YTTDPTVRRE 180
ECSVPVCGQE GRTTVVMTPR SGGSKDNLSP PLGQCLTERG RLYQGNLAVT TLGSPCLPWN 240
SLPAKTLSKY QDFDPEVKLV ENFCRNPDWD EEGAWCYVAG QPGDFEYCNL NYCEEAVGEE 300
NYDVDESIAG RTTDAEFHTF FNEKTFGLGE ADCGLRPLFE KKSLKDTTEK ELLDSYIDGR 360
IVEGWDAEKG IAPWQVMLFR KSPQELLCGA SLISDRWVLT AAHCILYPPW DKNFTENDLL 420
VRIGKHSRTR YERNVEKISM LEKIYVHPRY NWRENLDRDI ALLKLKKPVP FSDYIHPVCL 480
PDKQTVTSLL RAGYKGRVTG WGNLRETWTT NINEIQPSVL QVVNLPIVER PVCKASTRIR 540
ITDNMFCAGF KVNDTKRGDA CEGDSGGPFV MKSPFNNRWY QMGIVSWGEG CDRKGKYGFY 600
THVFRLKRWI QKVIDQFG 618 
Gene Ontology
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004252; F:serine-type endopeptidase activity; IDA:MGI.
 GO:0070053; F:thrombospondin receptor activity; IEA:Compara.
 GO:0006953; P:acute-phase response; IEA:UniProtKB-KW.
 GO:0051480; P:cytosolic calcium ion homeostasis; IEA:Compara.
 GO:0042730; P:fibrinolysis; IEA:Compara.
 GO:0048712; P:negative regulation of astrocyte differentiation; IEA:Compara.
 GO:0045861; P:negative regulation of proteolysis; IEA:Compara.
 GO:0030168; P:platelet activation; IDA:MGI.
 GO:0030194; P:positive regulation of blood coagulation; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IGI:MGI.
 GO:0008284; P:positive regulation of cell proliferation; IGI:MGI.
 GO:0032967; P:positive regulation of collagen biosynthetic process; IEA:Compara.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IGI:MGI.
 GO:1900738; P:positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IEA:Compara.
 GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0008360; P:regulation of cell shape; IGI:MGI.
 GO:0010468; P:regulation of gene expression; IDA:MGI.
 GO:0070493; P:thrombin receptor signaling pathway; ISO:MGI. 
Interpro
 IPR017857; Coagulation_fac_subgr_Gla_dom.
 IPR000294; GLA_domain.
 IPR000001; Kringle.
 IPR013806; Kringle-like.
 IPR018056; Kringle_CS.
 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR003966; Prothrombin/thrombin.
 IPR018992; Thrombin_light_chain.
 IPR009003; Trypsin-like_Pept_dom. 
Pfam
 PF00594; Gla
 PF00051; Kringle
 PF09396; Thrombin_light
 PF00089; Trypsin 
SMART
 SM00069; GLA
 SM00130; KR
 SM00020; Tryp_SPc 
PROSITE
 PS00011; GLA_1
 PS50998; GLA_2
 PS00021; KRINGLE_1
 PS50070; KRINGLE_2
 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 
PRINTS
 PR00722; CHYMOTRYPSIN.
 PR00001; GLABLOOD.
 PR01505; PROTHROMBIN.