CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023498
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitotic spindle assembly checkpoint protein MAD1 
Protein Synonyms/Alias
 Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1 
Gene Name
 Mad1l1 
Gene Synonyms/Alias
 Mad1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
61RAEQIRSKSYLIQVEacetylation[1, 2]
673ATGPSGSKMQLLETEubiquitination[3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding (By similarity). 
Sequence Annotation
 REGION 380 532 Necessary for interaction with NEK2 (By
 MOD_RES 16 16 Phosphoserine (By similarity).
 MOD_RES 61 61 N6-acetyllysine (By similarity).
 MOD_RES 214 214 Phosphoserine (By similarity).
 MOD_RES 428 428 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 717 AA 
Protein Sequence
MEDLGENTTV LSSLRSLNNF ISQRMEGTSG LDVSTSASGS LQKQYEYHMQ LEERAEQIRS 60
KSYLIQVERE KMQMELSHKR ARVELERAAS TNARNYEREV DRNQELLARI RQLQECEATA 120
EEKMREQLER HRLCKQNLDA VSQQLREQED SLASAREMIS SLKGRVSELQ LSAMDQKVQV 180
KRLESEKQEL KEQLELQQRK WQEANQKIQE LQASQDERAE HEQKIKDLEQ KLCLQEQDAA 240
VVKSMKSELM RMPRMERELK RLHEENTHLR EMKETNGLLT EELEGLQRKL SRQEKMQEAL 300
VDLELEKEKL LAKLQSWENL DQTMGLNLRT PEDLSRFVVE LQQRELTLKE KNNSITSSAR 360
GLEKVQQQLQ DEVRQANAQL LEERKKRETH EALARRLQKR NALLTKERDG MRAILGSYDS 420
ELTQTEYSTQ LTQRLWEAED MVQKVHAHSS EMEAQLSQAL EELGVQKQRA DTLEMELKML 480
KAQTSSAESS FSFCKEEVDA LRLKVEELEG ERSRLEQEKQ VLEMQMEKLT LQGDYNQSRT 540
KVLHMSLNPI SMARQRQHED HDRLQEECER LRGLVHALER GGPIPADLEA ASSLPSSKEV 600
AELRKQVESA ELKNQRLKEV FQTKIQEFRK VCYTLTGYQI DVTTESQYRL TSRYAEHQTD 660
CLIFKATGPS GSKMQLLETE FSRSVPELIE LHLLQQDSIP AFLSALTIEL FSRQTSI 717 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0000776; C:kinetochore; ISS:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0072686; C:mitotic spindle; ISS:UniProtKB.
 GO:0005643; C:nuclear pore; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007094; P:mitotic spindle assembly checkpoint; IEA:InterPro.
 GO:0090235; P:regulation of metaphase plate congression; ISS:UniProtKB. 
Interpro
 IPR008672; Mad1. 
Pfam
 PF05557; MAD 
SMART
  
PROSITE
  
PRINTS