CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002036
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lactose operon repressor 
Protein Synonyms/Alias
  
Gene Name
 lacI 
Gene Synonyms/Alias
 b0345; JW0336 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
2******MKPVTLYDVacetylation[1]
33QASHVSAKTREKVEAacetylation[1]
203LRLAGWHKYLTRNQIacetylation[1, 2]
325LLPVSLVKRKTTLAPacetylation[1, 2]
327PVSLVKRKTTLAPNTacetylation[1]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Repressor of the lactose operon. Binds allolactose as an inducer. 
Sequence Annotation
 DOMAIN 1 58 HTH lacI-type.
 DNA_BIND 6 25 H-T-H motif.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; DNA-binding; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 360 AA 
Protein Sequence
MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ 60
SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS 120
GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ 180
QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT 240
AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS 300
VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ 360 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR026908; LacI.
 IPR010982; Lambda_DNA-bd_dom.
 IPR000843; Tscrpt_reg_HTH_LacI. 
Pfam
 PF00356; LacI 
SMART
 SM00354; HTH_LACI 
PROSITE
 PS00356; HTH_LACI_1
 PS50932; HTH_LACI_2 
PRINTS
 PR00036; HTHLACI.