CPLM-011062

Genbank Nucleotide ID

Histone acetyltransferase GCN5

Protein Synonyms/Alias

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
25	DPEVKRVKLENNVEE	sumoylation	[1]
42	PEQAETNKQEGTDKE	acetylation	[2]

[1] Sumoylation of the yeast Gcn5 protein.
Sterner DE, Nathan D, Reindle A, Johnson ES, Berger SL.
Biochemistry. 2006 Jan 24;45(3):1035-42. [PMID: 16411780]
[2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]

Functional Description

Acetylates histone H2B to form H2BK11ac and H2BK16ac, histone H3 to form H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac and H3K36ac, with a lower preference histone H4 to form H4K8ac and H4K16ac, and contributes to H2A.Z acetylation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Operates in concert with certain DNA-binding transcriptional activators such as GCN4 or HAP2/3/4. Its acetyltransferase activity seems to be dependent on the association in different multisubunit complexes. Functions as histone acetyltransferase component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. The ADA histone acetyltransferase complex preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B, leading to transcription regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.

DOMAIN 100 255 N-acetyltransferase.
DOMAIN 344 414 Bromo.

3D-structure; Activator; Acyltransferase; Bromodomain; Chromatin regulator; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:SGD.
GO:0000775; C:chromosome, centromeric region; IDA:SGD.
GO:0005829; C:cytosol; IDA:SGD.
GO:0000124; C:SAGA complex; IDA:SGD.
GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD.
GO:0010484; F:H3 histone acetyltransferase activity; IDA:SGD.
GO:0070577; F:histone acetyl-lysine binding; IDA:SGD.
GO:0003713; F:transcription coactivator activity; TAS:SGD.
GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR016181; Acyl_CoA_acyltransferase.
IPR001487; Bromodomain.
IPR018359; Bromodomain_CS.
IPR000182; GNAT_dom.

PF00583; Acetyltransf_1
PF00439; Bromodomain

PS00633; BROMODOMAIN_1
PS50014; BROMODOMAIN_2
PS51186; GNAT

PR00503; BROMODOMAIN.