UniProt Accession

 NARJ_ECOLI; P0AF26; P11351 

Genbank Protein ID

 M20147; U00096; AP009048; X16181 

Genbank Nucleotide ID

 AAA24196.1; AAC74310.1; BAA36096.1; CAA34305.1 

Protein Name

 Nitrate reductase molybdenum cofactor assembly chaperone NarJ 

Protein Synonyms/Alias

 Redox enzyme maturation protein NarJ 

Gene Name

 narJ 

Gene Synonyms/Alias

 b1226; JW1217 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
37	AASKNLPKEDAHALG	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane. 

Sequence Annotation

  

Keyword

 Chaperone; Complete proteome; Cytoplasm; Nitrate assimilation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 236 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016530; F:metallochaperone activity; IMP:EcoCyc.
 GO:0051131; P:chaperone-mediated protein complex assembly; IDA:UniProtKB.
 GO:0042128; P:nitrate assimilation; IDA:UniProtKB. 

Interpro

 IPR020945; DMSO/NO3_reduct_chaperone.
 IPR003765; NO3_reductase_chaperone_NarJ. 

Pfam

 PF02613; Nitrate_red_del 

SMART

  

PROSITE

  

PRINTS