CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009929
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit beta-1 
Protein Synonyms/Alias
 Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; SCG 
Gene Name
 Kpnb1 
Gene Synonyms/Alias
 Impnb 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
23LELEAAQKFLERAAVacetylation[1]
23LELEAAQKFLERAAVubiquitination[2]
62VAAGLQIKNSLTSKDubiquitination[2]
68IKNSLTSKDPDIKAQubiquitination[2]
183AIIQGMRKEEPSNNVacetylation[3]
206LNSLEFTKANFDKESacetylation[3]
376PFIKEHIKNPDWRYRubiquitination[2]
537ESLMEIVKNSAKDCYacetylation[3]
537ESLMEIVKNSAKDCYubiquitination[2]
541EIVKNSAKDCYPAVQacetylation[4]
541EIVKNSAKDCYPAVQsuccinylation[4]
651LKYMEAFKPFLGIGLacetylation[3]
730AIGGEFKKYLEVVLNacetylation[3]
835AFGKDVLKLVEARPMacetylation[3]
835AFGKDVLKLVEARPMubiquitination[2]
867TLATWATKELRKLKNacetylation[1, 3, 4]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus (By similarity). 
Sequence Annotation
 DOMAIN 21 101 Importin N-terminal.
 REPEAT 124 163 HEAT 1.
 REPEAT 168 207 HEAT 2.
 REPEAT 213 250 HEAT 3.
 REPEAT 317 357 HEAT 4.
 REPEAT 362 399 HEAT 5.
 REPEAT 404 441 HEAT 6.
 REPEAT 602 641 HEAT 7.
 REPEAT 687 726 HEAT 8.
 REGION 329 342 IAB-binding.
 REGION 334 419 Ran-GTP binding (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 12 12 Phosphoserine (By similarity).
 MOD_RES 211 211 N6-acetyllysine; alternate (By
 MOD_RES 835 835 N6-acetyllysine (By similarity).
 MOD_RES 867 867 N6-acetyllysine (By similarity).
 CROSSLNK 206 206 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 211 211 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; ADP-ribosylation; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 876 AA 
Protein Sequence
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS QVARVAAGLQ 60
IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG TETYRPSSAS QCVAGIACAE 120
IPVSQWPELI PQLVANVTNP NSTEHMKEST LEAIGYICQD IDPEQLQDKS NEILTAIIQG 180
MRKEEPSNNV KLAATNALLN SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN 240
LVKIMSLYYQ YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA 300
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV CLMLLSTCCE 360
DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GSILEGPEPN QLKPLVIQAM PTLIELMKDP 420
SVVVRDTTAW TVGRICELLP EAAINDVYLA PLLQCLIEGL SAEPRVASNV CWAFSSLAEA 480
AYEAADVADD QEEPATYCLS SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA 540
KDCYPAVQKT TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA 600
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF KPFLGIGLKN 660
YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EVMQLLLENL GNENVHRSVK PQILSVFGDI 720
ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS DFDMVDYLNE LRESCLEAYT GIVQGLKGDQ 780
ENVHPDVMLV QPRVEFILSF IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR 840
PMIHELLTEG RRSKTNKAKT LATWATKELR KLKNQA 876 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0031965; C:nuclear membrane; IEA:Compara.
 GO:0005643; C:nuclear pore; IEA:InterPro.
 GO:0043234; C:protein complex; IDA:MGI.
 GO:0008565; F:protein transporter activity; IDA:MGI.
 GO:0006610; P:ribosomal protein import into nucleus; IDA:MGI. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021133; HEAT_type_2.
 IPR001494; Importin-beta_N.
 IPR027140; KPNB1. 
Pfam
 PF03810; IBN_N 
SMART
 SM00913; IBN_N 
PROSITE
 PS50077; HEAT_REPEAT
 PS50166; IMPORTIN_B_NT 
PRINTS