UniProt Accession

 GPD2_YEAST; P41911; D6W208; P50905 

Genbank Protein ID

 Z35169; X91067; Z74801; AY558560; BK006948 

Genbank Nucleotide ID

 CAA84532.1; CAA62526.1; CAA99068.1; AAS56886.1; DAA10724.1 

Protein Name

 Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 GPD2 

Gene Synonyms/Alias

 GPD3; YOL059W; O1222 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
80	AVSIVHLKRAPFKVT	acetylation	[1]
260	KDYQGDGKDVDHKIL	acetylation	[1]
369	KVATYMAKTGKSALE	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the production of glycerol under anaerobic growth conditions. Glycerol production serves as a redox sink by consuming the excess cytosolic NADH during anaerobic metabolism. 

Sequence Annotation

 NP_BIND 90 95 NAD (By similarity).
 REGION 359 360 Substrate binding (By similarity).
 ACT_SITE 294 294 Proton acceptor (By similarity).
 BINDING 178 178 NAD (By similarity).
 BINDING 201 201 NAD; via amide nitrogen (By similarity).
 BINDING 201 201 Substrate (By similarity).
 BINDING 234 234 NAD; via amide nitrogen (By similarity).
 BINDING 359 359 NAD (By similarity).
 BINDING 388 388 NAD (By similarity).
 MOD_RES 72 72 Phosphoserine.
 MOD_RES 75 75 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 440 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:SGD.
 GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IMP:SGD.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006071; P:glycerol metabolic process; IMP:SGD.
 GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
 GO:0006116; P:NADH oxidation; IMP:SGD. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR006168; G3P_DH_NAD-dep.
 IPR006109; G3P_DH_NAD-dep_C.
 IPR017751; G3P_DH_NAD-dep_euk.
 IPR011128; G3P_DH_NAD-dep_N.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF07479; NAD_Gly3P_dh_C
 PF01210; NAD_Gly3P_dh_N 

SMART

  

PROSITE

 PS00957; NAD_G3PDH 

PRINTS

 PR00077; GPDHDRGNASE.