UniProt Accession

 TMED4_HUMAN; Q7Z7H5; A4D2K8; B4DFJ4; Q56VW3; Q7Z432; Q8N2P6 

Genbank Protein ID

 AK074557; AK290125; AK294122; BX647965; CH236960; CH471128; CH471128; BC052641; BC057851; AY191223; AB097009 

Genbank Nucleotide ID

 BAC11058.1; BAF82814.1; BAG57455.1; EAL23751.1; EAW61090.1; EAW61091.1; AAH52641.1; AAH57851.1; AAP20105.1; BAC77362.1 

Protein Name

 Transmembrane emp24 domain-containing protein 4 

Protein Synonyms/Alias

 Endoplasmic reticulum stress-response protein 25; ERS25; GMP25iso; Putative NF-kappa-B-activating protein 156; p24 family protein alpha-3; p24alpha3 

Gene Name

 TMED4 

Gene Synonyms/Alias

 ERS25 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
63	YRTQMWDKQKEVFLP	ubiquitination	[1, 2]
65	TQMWDKQKEVFLPST	ubiquitination	[2]
82	LGMHVEVKDPDGKVV	ubiquitination	[2]
87	EVKDPDGKVVLSRQY	ubiquitination	[1]
128	MALFAGGKLRVHLDI	ubiquitination	[2, 3]
150	NYPEIAAKDKLTELQ	ubiquitination	[1]
152	PEIAAKDKLTELQLR	ubiquitination	[1, 2, 3, 4]
172	DQVEQIQKEQDYQRY	ubiquitination	[1, 2, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis (By similarity). 

Sequence Annotation

 DOMAIN 39 137 GOLD.
 MOTIF 220 227 COPI vesicle coat-binding (Potential).
 MOTIF 220 221 COPII vesicle coat-binding (Potential).
 CARBOHYD 117 117 N-linked (GlcNAc...).  

Keyword

 Alternative splicing; Coiled coil; Complete proteome; Endoplasmic reticulum; Glycoprotein; Membrane; Protein transport; Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR009038; GOLD. 

Pfam

 PF01105; EMP24_GP25L 

SMART

  

PROSITE

 PS50866; GOLD 

PRINTS