UniProt Accession

 ACOX1_HUMAN; Q15067; A8K6X8; A8KAA0; B4DK61; F5GYQ8; Q12863; Q15068; Q15101; Q16131; Q7Z3W5; Q9UD31 

Genbank Protein ID

 U03268; U03254; U03255; U03256; U03258; U03259; U03260; U03261; U03263; U03264; U03265; U03266; U03267; U03268; U03254; U03255; U03257; U03258; U03259; U03260; U03261; U03263; U03264; U03265; U03266; U03267; U07866; X71440; S69189; AK291793; AK292965; AK296409; BX537380; AC040980; AC087289; CH471099; BC008767; BC010425 

Genbank Nucleotide ID

 AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19113.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA19114.1; AAA18595.1; CAA50574.1; AAB30019.2; BAF84482.1; BAF85654.1; BAG59073.1; CAD97622.1; EAW89351.1; AAH08767.1; AAH10425.1 

Protein Name

 Peroxisomal acyl-coenzyme A oxidase 1 

Protein Synonyms/Alias

 AOX; Palmitoyl-CoA oxidase; Straight-chain acyl-CoA oxidase; SCOX 

Gene Name

 ACOX1 

Gene Synonyms/Alias

 ACOX 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
29	ILDGSPEKTRRRREI	ubiquitination	[1]
122	GTTAQKEKWLLSSKG	ubiquitination	[1]
198	AQLITKGKCYGLHAF	acetylation	[2]
216	IREIGTHKPLPGITV	acetylation	[3]
216	IREIGTHKPLPGITV	ubiquitination	[1, 2]
255	PRENMLMKYAQVKPD	acetylation	[3, 4, 5]
267	KPDGTYVKPLSNKLT	acetylation	[2, 3, 4, 5]
295	EAARALSKACTIAIR	ubiquitination	[1, 2]
437	QTARFLMKSYDQVHS	acetylation	[2, 3, 4, 5]
500	RLVEIAAKNLQKEVI	acetylation	[3, 4, 5]
504	IAAKNLQKEVIHRKS	acetylation	[2, 3, 4]
651	AEVHESYKHLKSLQS	acetylation	[4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 

Functional Description

 Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA. 

Sequence Annotation

 MOTIF 658 660 Microbody targeting signal.
 ACT_SITE 421 421 Proton acceptor (By similarity).
 BINDING 139 139 FAD (By similarity).
 BINDING 178 178 FAD; via amide nitrogen (By similarity).
 MOD_RES 26 26 Phosphoserine.
 MOD_RES 255 255 N6-acetyllysine.
 MOD_RES 267 267 N6-acetyllysine.
 MOD_RES 310 310 Phosphoserine (By similarity).
 MOD_RES 437 437 N6-acetyllysine.
 MOD_RES 500 500 N6-acetyllysine.
 MOD_RES 504 504 N6-acetyllysine.
 MOD_RES 643 643 N6-acetyllysine (By similarity).
 MOD_RES 649 649 Phosphoserine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Disease mutation; FAD; Fatty acid metabolism; Flavoprotein; Lipid metabolism; Oxidoreductase; Peroxisome; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 660 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005782; C:peroxisomal matrix; TAS:Reactome.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:InterPro.
 GO:0003997; F:acyl-CoA oxidase activity; IDA:UniProtKB.
 GO:0071949; F:FAD binding; IDA:UniProtKB.
 GO:0005504; F:fatty acid binding; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0016401; F:palmitoyl-CoA oxidase activity; IDA:UniProtKB.
 GO:0036109; P:alpha-linolenic acid metabolic process; TAS:Reactome.
 GO:0033540; P:fatty acid beta-oxidation using acyl-CoA oxidase; IMP:BHF-UCL.
 GO:0019395; P:fatty acid oxidation; IGI:UniProtKB.
 GO:0006091; P:generation of precursor metabolites and energy; IMP:UniProtKB.
 GO:0055088; P:lipid homeostasis; IDA:UniProtKB.
 GO:0016559; P:peroxisome fission; IGI:UniProtKB.
 GO:2000189; P:positive regulation of cholesterol homeostasis; IGI:UniProtKB.
 GO:0006693; P:prostaglandin metabolic process; IMP:UniProtKB.
 GO:0007283; P:spermatogenesis; IEA:Compara.
 GO:0000038; P:very long-chain fatty acid metabolic process; IMP:BHF-UCL. 

Interpro

 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR012258; Acyl-CoA_oxidase.
 IPR002655; Acyl-CoA_oxidase_C.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 

Pfam

 PF01756; ACOX
 PF02770; Acyl-CoA_dh_M 

SMART

  

PROSITE

  

PRINTS