CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020254
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase RIO2 
Protein Synonyms/Alias
 RIO kinase 2 
Gene Name
 RIOK2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
86GYDYLALKTLSSRQVubiquitination[1, 2, 3]
160LSRLSAMKEFAYMKAubiquitination[3]
270DRDVKCIKDFFMKRFubiquitination[3]
275CIKDFFMKRFSYESEubiquitination[3]
288SELFPTFKDIRREDTubiquitination[1, 2, 3, 4]
377SGDPEQIKEDSLSEEubiquitination[1]
523AVRRRLQKGEANIFTubiquitination[3]
531GEANIFTKQRRENMQubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
 DOMAIN 271 552 Protein kinase.
 ACT_SITE 228 228 Proton acceptor (By similarity).
 BINDING 123 123 ATP (By similarity).
 MOD_RES 332 332 Phosphoserine.
 MOD_RES 335 335 Phosphoserine.
 MOD_RES 337 337 Phosphoserine.
 MOD_RES 350 350 Phosphoserine.
 MOD_RES 362 362 Phosphoserine.
 MOD_RES 380 380 Phosphoserine.
 MOD_RES 382 382 Phosphoserine.
 MOD_RES 385 385 Phosphoserine.
 MOD_RES 390 390 Phosphoserine.
 MOD_RES 412 412 Phosphoserine.
 MOD_RES 417 417 Phosphoserine.
 MOD_RES 442 442 Phosphoserine.
 MOD_RES 445 445 Phosphotyrosine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 552 AA 
Protein Sequence
MGKVNVAKLR YMSRDDFRVL TAVEMGMKNH EIVPGSLIAS IASLKHGGCN KVLRELVKHK 60
LIAWERTKTV QGYRLTNAGY DYLALKTLSS RQVVESVGNQ MGVGKESDIY IVANEEGQQF 120
ALKLHRLGRT SFRNLKNKRD YHKHRHNVSW LYLSRLSAMK EFAYMKALYE RKFPVPKPID 180
YNRHAVVMEL INGYPLCQIH HVEDPASVYD EAMELIVKLA NHGLIHGDFN EFNLILDESD 240
HITMIDFPQM VSTSHPNAEW YFDRDVKCIK DFFMKRFSYE SELFPTFKDI RREDTLDVEV 300
SASGYTKEMQ ADDELLHPLG PDDKNIETKE GSEFSFSDGE VAEKAEVYGS ENESERNCLE 360
ESEGCYCRSS GDPEQIKEDS LSEESADARS FEMTEFNQAL EEIKGQVVEN NSVTEFSEEK 420
NRTENYNRQD GQRVQGGVPA GSDEYEDECP HLIALSSLNR EFRPFRDEEN VGAMNQYRTR 480
TLSITSSGSA VSCSTIPPEL VKQKVKRQLT KQQKSAVRRR LQKGEANIFT KQRRENMQNI 540
KSSLEAASFW GE 552 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR018934; RIO-like_kinase.
 IPR015285; RIO2_kinase_winged_hlx_N.
 IPR018935; RIO_kinase_CS.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF01163; RIO1
 PF09202; Rio2_N 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS01245; RIO1 
PRINTS