CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029848
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 TNF receptor-associated factor 2 
Protein Synonyms/Alias
  
Gene Name
 TRAF2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
234VKRLDQDKIEALSSKubiquitination[1, 2, 3]
241KIEALSSKVQQLERSubiquitination[1, 2, 3, 4, 5, 6]
252LERSIGLKDLAMADLubiquitination[1, 2, 3, 5, 6]
398PLFCPVSKMEAKNSYubiquitination[4]
402PVSKMEAKNSYVRDDubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 422 AA 
Protein Sequence
MAAASVTPPG SLELLQPGFS KTLLGTKLEA KYLCSACRNV LRRPFQAQCG HRYCSFCLAS 60
ILSSGPQNCA ACVHEGIYEE GISILESSSA FPDNAARREV ESLPAVCPSD GCTWKGTLKE 120
YEVKMPACGM VTEAPAVGSR PRSPSSYDLV LHVPLTGAEA CLMSVEEETE LLLRSCHEGR 180
CPLMLTECPA CKGLVRLGEK ERHLEHECPE RSLSCRHCRA PCCGADVKRL DQDKIEALSS 240
KVQQLERSIG LKDLAMADLE QKVLEMEAST YDGVFIWKIS DFARKRQEAV AGRIPAIFSP 300
AFYTSRYGYK MCLRIYLNGD GTGRGTHLSL FFVVMKGPND ALLRWPFNQK VTLMLLDQNN 360
REHVIDAFRP DVTSSSFQRP VNDMNIASGC PLFCPVSKME AKNSYVRDDA IFIKAIVDLT 420
GL 422 
Gene Ontology
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007250; P:activation of NF-kappaB-inducing kinase activity; IEA:InterPro.
 GO:0051865; P:protein autoubiquitination; IEA:InterPro.
 GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
 GO:0046328; P:regulation of JNK cascade; IEA:InterPro.
 GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IEA:InterPro. 
Interpro
 IPR002083; MATH.
 IPR012227; TNF_rcpt--assoc_TRAF.
 IPR008974; TRAF-like.
 IPR027133; TRAF2.
 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS.
 IPR001293; Znf_TRAF. 
Pfam
 PF00917; MATH
 PF00097; zf-C3HC4 
SMART
 SM00061; MATH
 SM00184; RING 
PROSITE
 PS50144; MATH
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2
 PS50145; ZF_TRAF 
PRINTS