CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022526
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 23 
Protein Synonyms/Alias
 Rho-type GTPase-activating protein 23 
Gene Name
 ARHGAP23 
Gene Synonyms/Alias
 KIAA1501 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
674EDADAPSKRHSTSDLubiquitination[1]
824ANQALISKKLNDYRKubiquitination[2]
825NQALISKKLNDYRKVubiquitination[1]
854SRGLGGLKSEFLKQSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state (By similarity). 
Sequence Annotation
 DOMAIN 71 155 PDZ.
 DOMAIN 688 808 PH.
 DOMAIN 905 1097 Rho-GAP.
 MOD_RES 351 351 Phosphoserine (By similarity).
 MOD_RES 423 423 Phosphoserine.
 MOD_RES 598 598 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1491 AA 
Protein Sequence
MNGVAFCLVG IPPRPEPRPP QLPLGPRDGC SPRRPFPWQG PRTLLLYKSP QDGFGFTLRH 60
FIVYPPESAV HCSLKEEENG GRGGGPSPRY RLEPMDTIFV KNVKEDGPAH RAGLRTGDRL 120
VKVNGESVIG KTYSQVIALI QNSDDTLELS IMPKDEDILQ LAYSQDAYLK GNEPYSGEAR 180
SIPEPPPICY PRKTYAPPAR ASTRATMVPE PTSALPSDPR SPAAWSDPGL RVPPAARAHL 240
DNSSLGMSQP RPSPGAFPHL SSEPRTPRAF PEPGSRVPPS RLECQQALSH WLSNQVPRRA 300
GERRCPAMAP RARSASQDRL EEVAAPRPWP CSTSQDALSQ LGQEGWHRAR SDDYLSRATR 360
SAEALGPGAL VSPRFERCGW ASQRSSARTP ACPTRDLPGP QAPPPSGLQG LDDLGYIGYR 420
SYSPSFQRRT GLLHALSFRD SPFGGLPTFN LAQSPASFPP EASEPPRVVR PEPSTRALEP 480
PAEDRGDEVV LRQKPPTGRK VQLTPARQMN LGFGDESPEP EASGRGERLG RKVAPLATTE 540
DSLASIPFID EPTSPSIDLQ AKHVPASAVV SSAMNSAPVL GTSPSSPTFT FTLGRHYSQD 600
CSSIKAGRRS SYLLAITTER SKSCDDGLNT FRDEGRVLRR LPNRIPSLRM LRSFFTDGSL 660
DSWGTSEDAD APSKRHSTSD LSDATFSDIR REGWLYYKQI LTKKGKKAGS GLRQWKRVYA 720
ALRARSLSLS KERREPGPAA AGAAAAGAGE DEAAPVCIGS CLVDISYSET KRRHVFRLTT 780
ADFCEYLFQA EDRDDMLGWI RAIRENSRAE GEDPGCANQA LISKKLNDYR KVSHSSGPKA 840
DSSPKGSRGL GGLKSEFLKQ SAARGLRTQD LPAGSKDDSA AAPKTPWGIN IIKKNKKAAP 900
RAFGVRLEEC QPATENQRVP LIVAACCRIV EARGLESTGI YRVPGNNAVV SSLQEQLNRG 960
PGDINLQDER WQDLNVISSL LKSFFRKLPE PLFTDDKYND FIEANRIEDA RERMRTLRKL 1020
IRDLPGHYYE TLKFLVGHLK TIADHSEKNK MEPRNLALVF GPTLVRTSED NMTDMVTHMP 1080
DRYKIVETLI QHSDWFFSDE EDKGERTPVG DKEPQAVPNI EYLLPNIGRT VPPGDPGSDS 1140
TTCSSAKSKG SWAPKKEPYA REMLAISFIS AVNRKRKKRR EARGLGSSTD DDSEQEAHKP 1200
GAGATAPGTQ ERPQGPLPGA VAPEAPGRLS PPAAPEERPA ADTRSIVSGY STLSTMDRSV 1260
CSGASGRRAG AGDEADDERS ELSHVETDTE GAAGAGPGGR LTRRPSFSSH HLMPCDTLAR 1320
RRLARGRPDG EGAGRGGPRA PEPPGSASSS SQESLRPPAA ALASRPSRME ALRLRLRGTA 1380
DDMLAVRLRR PLSPETRRRR SSWRRHTVVV QSPLTDLNFN EWKELGGGGP PEPAGARAHS 1440
DNKDSGLSSL ESTKARAPSS AASQPPAPGD TGSLQSQPPR RSAASRLHQC L 1491 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR001478; PDZ.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00595; PDZ
 PF00169; PH
 PF00620; RhoGAP 
SMART
 SM00228; PDZ
 SM00233; PH
 SM00324; RhoGAP 
PROSITE
 PS50106; PDZ
 PS50003; PH_DOMAIN
 PS50238; RHOGAP 
PRINTS