CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000804
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peroxisome biogenesis factor 1 
Protein Synonyms/Alias
 Peroxin-1; Peroxisome biogenesis disorder protein 1 
Gene Name
 PEX1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
211RDQKGMMKELQTKQLubiquitination[1]
260FSFQSEKKQETSWGLubiquitination[1]
274LTEINAFKNMQSKVVubiquitination[1]
279AFKNMQSKVVPLDNIubiquitination[1]
291DNIFRVCKSQPPSIYubiquitination[1]
337VTYGKLVKLLSPKQQubiquitination[1]
342LVKLLSPKQQQSKTKubiquitination[1]
349KQQQSKTKQNVLSPEubiquitination[1]
357QNVLSPEKEKQMSEPubiquitination[1]
402NNAIKYTKNVEVLHLubiquitination[1]
411VEVLHLGKVWIPDDLubiquitination[1]
455QPRENLPKDISEEDIubiquitination[1]
511VHSWEKEKDKNIFLLubiquitination[1]
513SWEKEKDKNIFLLSPubiquitination[1]
601ALLLTGGKGSGKSTLubiquitination[1]
605TGGKGSGKSTLAKAIubiquitination[1]
614TLAKAICKEAFDKLDubiquitination[1]
619ICKEAFDKLDAHVERubiquitination[1]
630HVERVDCKALRGKRLubiquitination[1]
756LCNVIKNKLDCDINKubiquitination[1]
763KLDCDINKFTDLDLQubiquitination[1]
806QSISTREKLVLTTLDubiquitination[2, 3]
816LTTLDFQKALRGFLPubiquitination[1]
833LRSVNLHKPRDLGWDubiquitination[1, 3, 4]
841PRDLGWDKIGGLHEVubiquitination[1]
887YGPPGTGKTLLAGVIubiquitination[1]
906RMNFISVKGPELLSKubiquitination[1]
913KGPELLSKYIGASEQubiquitination[1, 5]
933FIRAQAAKPCILFFDubiquitination[1]
1001LRPGRLDKCVYCPPPubiquitination[1]
1204RADISIIKGRYRSQSubiquitination[1]
1224MNQPGPIKTRLAISQubiquitination[1, 6]
1264YESFQNPKRRKNQSGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes. 
Sequence Annotation
 NP_BIND 599 606 ATP (Potential).
 NP_BIND 881 888 ATP (Potential).
 MOD_RES 1209 1209 Phosphoserine.
 MOD_RES 1211 1211 Phosphoserine (By similarity).
 CROSSLNK 833 833 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Disease mutation; Isopeptide bond; Membrane; Nucleotide-binding; Peroxisome; Peroxisome biogenesis; Peroxisome biogenesis disorder; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation; Zellweger syndrome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1283 AA 
Protein Sequence
MWGSDRLAGA GGGGAAVTVA FTNARDCFLH LPRRLVAQLH LLQNQAIEVV WSHQPAFLSW 60
VEGRHFSDQG ENVAEINRQV GQKLGLSNGG QVFLKPCSHV VSCQQVEVEP LSADDWEILE 120
LHAVSLEQHL LDQIRIVFPK AIFPVWVDQQ TYIFIQIVAL IPAASYGRLE TDTKLLIQPK 180
TRRAKENTFS KADAEYKKLH SYGRDQKGMM KELQTKQLQS NTVGITESNE NESEIPVDSS 240
SVASLWTMIG SIFSFQSEKK QETSWGLTEI NAFKNMQSKV VPLDNIFRVC KSQPPSIYNA 300
SATSVFHKHC AIHVFPWDQE YFDVEPSFTV TYGKLVKLLS PKQQQSKTKQ NVLSPEKEKQ 360
MSEPLDQKKI RSDHNEEDEK ACVLQVVWNG LEELNNAIKY TKNVEVLHLG KVWIPDDLRK 420
RLNIEMHAVV RITPVEVTPK IPRSLKLQPR ENLPKDISEE DIKTVFYSWL QQSTTTMLPL 480
VISEEEFIKL ETKDGLKEFS LSIVHSWEKE KDKNIFLLSP NLLQKTTIQV LLDPMVKEEN 540
SEEIDFILPF LKLSSLGGVN SLGVSSLEHI THSLLGRPLS RQLMSLVAGL RNGALLLTGG 600
KGSGKSTLAK AICKEAFDKL DAHVERVDCK ALRGKRLENI QKTLEVAFSE AVWMQPSVVL 660
LDDLDLIAGL PAVPEHEHSP DAVQSQRLAH ALNDMIKEFI SMGSLVALIA TSQSQQSLHP 720
LLVSAQGVHI FQCVQHIQPP NQEQRCEILC NVIKNKLDCD INKFTDLDLQ HVAKETGGFV 780
ARDFTVLVDR AIHSRLSRQS ISTREKLVLT TLDFQKALRG FLPASLRSVN LHKPRDLGWD 840
KIGGLHEVRQ ILMDTIQLPA KYPELFANLP IRQRTGILLY GPPGTGKTLL AGVIARESRM 900
NFISVKGPEL LSKYIGASEQ AVRDIFIRAQ AAKPCILFFD EFESIAPRRG HDNTGVTDRV 960
VNQLLTQLDG VEGLQGVYVL AATSRPDLID PALLRPGRLD KCVYCPPPDQ VSRLEILNVL 1020
SDSLPLADDV DLQHVASVTD SFTGADLKAL LYNAQLEALH GMLLSSGLQD GSSSSDSDLS 1080
LSSMVFLNHS SGSDDSAGDG ECGLDQSLVS LEMSEILPDE SKFNMYRLYF GSSYESELGN 1140
GTSSDLSSQC LSAPSSMTQD LPGVPGKDQL FSQPPVLRTA SQEGCQELTQ EQRDQLRADI 1200
SIIKGRYRSQ SGEDESMNQP GPIKTRLAIS QSHLMTALGH TRPSISEDDW KNFAELYESF 1260
QNPKRRKNQS GTMFRPGQKV TLA 1283 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005778; C:peroxisomal membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IMP:UniProtKB.
 GO:0042623; F:ATPase activity, coupled; IMP:UniProtKB.
 GO:0032403; F:protein complex binding; IDA:UniProtKB.
 GO:0060152; P:microtubule-based peroxisome localization; IMP:UniProtKB.
 GO:0016558; P:protein import into peroxisome matrix; IMP:UniProtKB. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR009010; Asp_de-COase-like_dom.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase.
 IPR015343; Peroxisome_synth_fac_1_a/b.
 IPR015342; PEX-1N.
 IPR025653; Pex1. 
Pfam
 PF00004; AAA
 PF09262; PEX-1N
 PF09263; PEX-2N 
SMART
 SM00382; AAA 
PROSITE
 PS00674; AAA 
PRINTS