UniProt Accession

 PHEA_ECOLI; P0A9J8; P07022; P78204 

Genbank Protein ID

 M10431; U00096; AP009048; M58024; V00314 

Genbank Nucleotide ID

 AAA24330.1; AAC75648.1; BAA16484.1; AAA62784.1; CAA23601.1 

Protein Name

 P-protein 

Protein Synonyms/Alias

 Chorismate mutase; CM; Prephenate dehydratase; PDT 

Gene Name

 pheA 

Gene Synonyms/Alias

 b2599; JW2580 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
20	KISALDEKLLALLAE	acetylation	[1]
39	AVEVGKAKLLSHRPV	acetylation	[1]
64	ERLITLGKAHHLDAH	acetylation	[1]
112	RIAFLGPKGSYSHLA	acetylation	[1]
227	LNRYPHWKIEYTEST	acetylation	[1]
240	STSAAMEKVAQAKSP	acetylation	[1]
286	RFVVLARKAINVSDQ	acetylation	[1, 2]
360	AEMQKALKELGEITR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate. 

Sequence Annotation

 DOMAIN 1 92 Chorismate mutase.
 DOMAIN 105 285 Prephenate dehydratase.
 DOMAIN 299 376 ACT.
 REGION 286 386 Regulatory (Phe-binding).
 BINDING 11 11 Substrate.
 BINDING 28 28 Substrate.
 BINDING 39 39 Substrate.
 BINDING 48 48 Substrate.
 BINDING 52 52 Substrate.
 BINDING 84 84 Substrate.
 BINDING 88 88 Substrate.  

Keyword

 3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Complete proteome; Cytoplasm; Isomerase; Lyase; Multifunctional enzyme; Phenylalanine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 386 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004106; F:chorismate mutase activity; IDA:EcoCyc.
 GO:0004664; F:prephenate dehydratase activity; IDA:EcoCyc.
 GO:0046417; P:chorismate metabolic process; IEA:InterPro.
 GO:0009094; P:L-phenylalanine biosynthetic process; IMP:EcoCyc.
 GO:0006571; P:tyrosine biosynthetic process; IMP:EcoCyc. 

Interpro

 IPR008242; Chor_mutase/pphenate_deHydtase.
 IPR002701; Chorismate_mutase.
 IPR020822; Chorismate_mutase_type_II.
 IPR010952; CM_P_1.
 IPR001086; Preph_deHydtase.
 IPR018528; Preph_deHydtase_CS. 

Pfam

 PF01817; CM_2
 PF00800; PDT 

SMART

 SM00830; CM_2 

PROSITE

 PS51671; ACT
 PS51168; CHORISMATE_MUT_2
 PS00857; PREPHENATE_DEHYDR_1
 PS00858; PREPHENATE_DEHYDR_2
 PS51171; PREPHENATE_DEHYDR_3 

PRINTS