CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039889
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 CDK5 regulatory subunit-associated protein 3 
Protein Synonyms/Alias
  
Gene Name
 CDK5RAP3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108KGTEASTKNIFGRYSubiquitination[1, 2]
156YEIPSLKKQIAKCQQubiquitination[1]
160SLKKQIAKCQQLQQEubiquitination[1, 2]
171LQQEYSRKEEECQAGubiquitination[1]
191EQFYHSCKQYGITGEubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
420AILQGQTKEKMVTMVubiquitination[4, 8]
465VTEFLQQKLKQSQLLubiquitination[1]
467EFLQQKLKQSQLLALubiquitination[1]
476SQLLALKKELMVQKQubiquitination[1]
482KKELMVQKQQEALEEubiquitination[1, 8]
496EQAALEPKLDLLLEKubiquitination[1]
503KLDLLLEKTKELQKLubiquitination[1, 4]
517LIEADISKRYSGRPVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MRRQSMTSAT RDLHTALDGK ATQGGKKDHQ HVPIDIQTSK LLDWLVDRRH CSLKWQSLVL 60
TIREKINAAI QDMPESEEIA QLLSGSYIHY FHCLRILDLL KGTEASTKNI FGRYSSQRMK 120
DWQEIIALYE KDNTYLVELS SLLVRNVNYE IPSLKKQIAK CQQLQQEYSR KEEECQAGAA 180
EMREQFYHSC KQYGITGENV RGELLALVKD LPSQLAEIGA AAQQSLGEAI DVYQASVGFV 240
CESPTEQVLP MLRFVQKRGN STVYEWRTGT EPSVVERPHL EELPEQVAED AIDWGDFGVE 300
AVSEGTDSGI SAEAAGIDWG IFPESDSKDP GGDGIDWGDD AVALQITVLE AGTQAPEGVA 360
RGPDALTLLE YTETRNQFLD ELMELEIFLA QRAVELSEEA DVLSVSQFQL APAILQGQTK 420
EKMVTMVSVL EDLIGKLTSL QLQHLFMILA SPRYVDRVTE FLQQKLKQSQ LLALKKELMV 480
QKQQEALEEQ AALEPKLDLL LEKTKELQKL IEADISKRYS GRPVNLMGTS L 531 
Gene Ontology
  
Interpro
 IPR008491; DUF773. 
Pfam
 PF05600; DUF773 
SMART
  
PROSITE
  
PRINTS