UniProt Accession

 EIF3H_HUMAN; O15372 

Genbank Protein ID

 U54559; AF092576; AF092569; AF092570; AF092571; AF092572; AF092573; AF092574; AF092575; BC000386 

Genbank Nucleotide ID

 AAD03465.1; AAC84044.1; AAC84044.1; AAC84044.1; AAC84044.1; AAC84044.1; AAC84044.1; AAC84044.1; AAC84044.1; AAH00386.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit H 

Protein Synonyms/Alias

 eIF3h; Eukaryotic translation initiation factor 3 subunit 3; eIF-3-gamma; eIF3 p40 subunit 

Gene Name

 EIF3H 

Gene Synonyms/Alias

 EIF3S3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MASRKEGTGSTA	ubiquitination	[1, 2]
24	STAGAAGKGKGKGGS	ubiquitination	[1]
165	AQGSLSLKAYRLTPK	ubiquitination	[1]
172	KAYRLTPKLMEVCKE	ubiquitination	[1]
178	PKLMEVCKEKDFSPE	ubiquitination	[1]
180	LMEVCKEKDFSPEAL	ubiquitination	[1]
188	DFSPEALKKANITFE	ubiquitination	[2]
189	FSPEALKKANITFEY	ubiquitination	[1]
220	VLMWELEKKSAVADK	ubiquitination	[1]
221	LMWELEKKSAVADKH	ubiquitination	[1]
227	KKSAVADKHELLSLA	ubiquitination	[1, 2]
241	ASSNHLGKNLQLLMD	ubiquitination	[3]
259	EMSQDIVKYNTYMRN	ubiquitination	[1, 2, 3]
303	LPEEDLSKLFKPPQP	ubiquitination	[1, 3]
306	EDLSKLFKPPQPPAR	ubiquitination	[1, 3]
340	FTAQNLGKLFMAQAL	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 DOMAIN 34 146 MPN.
 MOD_RES 183 183 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 352 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; TAS:UniProtKB.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR027524; eIF3h.
 IPR000555; JAB1_Mov34_MPN_PAD1. 

Pfam

 PF01398; JAB 

SMART

 SM00232; JAB_MPN 

PROSITE

  

PRINTS