CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011503
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA (guanosine(18)-2'-O)-methyltransferase 
Protein Synonyms/Alias
 tRNA [Gm18] methyltransferase; tRNA methylase 3 
Gene Name
 TRM3 
Gene Synonyms/Alias
 YDL112W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
209KLMRWHIKYLSKCCNacetylation[1]
1107DLRNDEKKYGFTQLLacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Specifically methylates guanosine-18 in various tRNAs. 
Sequence Annotation
 BINDING 1389 1389 S-adenosyl-L-methionine; via carbonyl
 BINDING 1409 1409 S-adenosyl-L-methionine; via amide  
Keyword
 Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1436 AA 
Protein Sequence
MVGGALICKY LPREEQLKLI SDLIQNDSLE EVLELIETSP LDITTDSNIE TPIFEKITEQ 60
VIAYASIDGE AREMFRSSRA EMNKALRTSA QLLCCLPSVW HKFQVWMSYR LNDIISENYK 120
HLFNDNFGKK IVQPFFDSFA EEQNANIKHE NLHLDILSLL HYLEVVYLFD ECKNGISSKC 180
LDFIIVPLLG CNSEEIADSC SKLMRWHIKY LSKCCNTDSN FDKLIWTFIK QLYAEGSQQA 240
WKQKNSLSFL LRFLLAAELS PELITYIKTD AYWRHIQTEL DNDVHEHRKL ALSILKLTIQ 300
KLSSHGITLQ TTFYKCNDLA NIEMLGSWKK FTTLYEMIAL DTSLNQIQAA KQDIIKIFDN 360
EHLHHSWGLI LLSTGLKSSM ESVRKYMMTL MFSITNMSAF SSNLPLLTKT LLPAAMSAHY 420
FDVKGVSCPH GEKLSLFVNN LLSQTTEGIS DILFEILKLL VEKGTSFDPS RIYLSYGILV 480
FFQNNKQKTI NSDHLSLIRK LYDFAAEEEV LETTIQTIYL KFLLYIDPSV SASELLFTLV 540
SHIKLKGGTY KYVEPLFEDY RDLAVSHFDD LQAKENLTTN IGKDTIFDLL ASIIFDFKDI 600
DITPDFLIEV AKSKQDIPVY TSKAVTFLTQ LLSGEPSNGY TYENATALLS YPNFTISTWK 660
SINVNNLFKS VMEKFSLDKF KFFAEIYQKT YECRFDTIEL NFNDLLSLYE MVKKSANQCS 720
RESFKVKDSA YSSYFELLNT FLKTYALNRD SSEGNDDELH ILLRLVDENI NKDNGNYLGN 780
LAVCKLLYFI IDSYIHCSTS VSDDDIFIVK FIFEKFSFIW ECINSERLVL KERDLHLMLI 840
KGLFHPVILY FGSNQYIDTL TSKLEEHAQT IISLSYSRRS LLPLLGSQLR VFMKFYGKLL 900
REDVNYWWLI NIIVGVFKQP QMDVNLYKLK PVISSLFDHK LNNYYIKGDE LYEKVYGPDE 960
ILARVSIIDS ILYANDQLKI RLIEKVTEKT NALYAIKRTD GAEALQRLLQ WQLLLLSLLT 1020
TNEKKLSETS MIRILKSIED ESSPLVRVYK EWFISSKVVD YYKTGNPKFA EDYLFSLLED 1080
HSKPVFVVSA EKICFMVLKD LRNDEKKYGF TQLLDRFICT LVPNAASNKP LVRHFSNSLI 1140
ISLWPTFKAY LSDHTLRNII ENLYSNAKKT QIFGQYRAGD ANIWDLKGDR KLTNMFGGVL 1200
KKVTDHDCPY ISESVFEKYL QEKDIVPIGT DERSLWLDKR DTNTESVNNA NISCDTSPLQ 1260
TKSGAWETVL DLDNKKSNDV VTRSELIVVS SLVDKPPNLG GICRLCDVLG VGLLTVQDIK 1320
VKNHPQFKNV AVTADRWMPM EEVALDEIAS FMKEKKKEGY TLIGLEQTDK SVKLDNNFQF 1380
PKKSLILLGT EAFGIPGTLL SELDLCLEIQ QFGVIRSMNI QTATAVIVHS YTVQHM 1436 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0016423; F:tRNA (guanine) methyltransferase activity; IDA:SGD.
 GO:0009020; F:tRNA (guanosine-2'-O-)-methyltransferase activity; IEA:EC. 
Interpro
 IPR001537; SpoU_MeTrfase.
 IPR025759; tRNA_mtfrase_TRM3. 
Pfam
 PF00588; SpoU_methylase 
SMART
  
PROSITE
  
PRINTS