CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002918
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 D-alanine--D-alanine ligase A 
Protein Synonyms/Alias
 D-Ala-D-Ala ligase A; D-alanylalanine synthetase A 
Gene Name
 ddlA 
Gene Synonyms/Alias
 b0381; JW0372 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
33NIVDAIDKSRFDVVLacetylation[1]
177SFAEVESKLGLPLFVacetylation[1]
225VIVEQGIKGREIECAacetylation[1]
267YIDEDGAKVVVPAAIacetylation[1]
281IAPEINDKIRAIAVQacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Cell wall formation. 
Sequence Annotation
 DOMAIN 145 348 ATP-grasp.
 NP_BIND 175 230 ATP (By similarity).
 METAL 302 302 Magnesium or manganese 1 (By similarity).
 METAL 315 315 Magnesium or manganese 1 (By similarity).
 METAL 315 315 Magnesium or manganese 2 (By similarity).
 METAL 317 317 Magnesium or manganese 2 (By similarity).  
Keyword
 ATP-binding; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Peptidoglycan synthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 364 AA 
Protein Sequence
MEKLRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH VSDASNYLLN 60
ADDPAHIALR PSATSLAQVP GKHEHQLIDA QNGQPLPTVD VIFPIVHGTL GEDGSLQGML 120
RVANLPFVGS DVLASAACMD KDVTKRLLRD AGLNIAPFIT LTRANRHNIS FAEVESKLGL 180
PLFVKPANQG SSVGVSKVTS EEQYAIAVDL AFEFDHKVIV EQGIKGREIE CAVLGNDNPQ 240
ASTCGEIVLT SDFYAYDTKY IDEDGAKVVV PAAIAPEIND KIRAIAVQAY QTLGCAGMAR 300
VDVFLTPENE VVINEINTLP GFTNISMYPK LWQASGLGYT DLITRLIELA LERHAADNAL 360
KTTM 364 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0008716; F:D-alanine-D-alanine ligase activity; IDA:EcoliWiki.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. 
Interpro
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR000291; D-Ala_lig_Van_CS.
 IPR005905; D_ala_D_ala.
 IPR011095; Dala_Dala_lig_C.
 IPR011127; Dala_Dala_lig_N.
 IPR016185; PreATP-grasp_dom. 
Pfam
 PF07478; Dala_Dala_lig_C
 PF01820; Dala_Dala_lig_N 
SMART
  
PROSITE
 PS50975; ATP_GRASP
 PS00843; DALA_DALA_LIGASE_1
 PS00844; DALA_DALA_LIGASE_2 
PRINTS