UniProt Accession

 THOC4_HUMAN; Q86V81; O43672 

Genbank Protein ID

 AC145207; BC052302; AF047002 

Genbank Nucleotide ID

 AAH52302.1; AAD09608.1 

Protein Name

 THO complex subunit 4 

Protein Synonyms/Alias

 Tho4; Ally of AML-1 and LEF-1; Aly/REF export factor; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF 

Gene Name

 ALYREF 

Gene Synonyms/Alias

 ALY; BEF; THOC4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
81	PAPYSRPKQLPDKWQ	ubiquitination	[1]
86	RPKQLPDKWQHDLFD	ubiquitination	[1, 2, 3]
164	ADALKAMKQYNGVPL	ubiquitination	[1, 2, 3]
235	RGAGRNSKQQLSAEE	ubiquitination	[2, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. ALYREF/THOC4 in conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. 

Sequence Annotation

 DOMAIN 106 183 RRM.
 REGION 54 155 Interaction with HHV-8 ORF57 protein.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 8 8 Phosphoserine.
 MOD_RES 94 94 Phosphoserine.
 MOD_RES 204 204 Dimethylated arginine; alternate.
 MOD_RES 204 204 Omega-N-methylated arginine; alternate.
 MOD_RES 220 220 Omega-N-methylarginine.
 MOD_RES 239 239 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Methylation; mRNA processing; mRNA splicing; mRNA transport; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 257 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000346; C:transcription export complex; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0046784; P:intronless viral mRNA export from host nucleus; IDA:UniProtKB.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR025715; FoP_duplication.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF13865; FoP_duplication
 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS