UniProt Accession

 SPC24_HUMAN; Q8NBT2; C9JGC4 

Genbank Protein ID

 AY456387; AK075287; AC011485; BC105037; BC105039 

Genbank Nucleotide ID

 AAR88651.1; BAC11523.1; AAI05038.1; AAI05040.1 

Protein Name

 Kinetochore protein Spc24 

Protein Synonyms/Alias

 hSpc24 

Gene Name

 SPC24 

Gene Synonyms/Alias

 SPBC24 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	ETQDGAEKQLREILT	ubiquitination	[1, 2, 3]
60	REILTMEKEVAQSLL	ubiquitination	[3, 4]
70	AQSLLNAKEQVHQGG	ubiquitination	[2, 3]
98	GEEDTRLKASLLYLT	ubiquitination	[2]
112	TRELEELKEIEADLE	ubiquitination	[2, 3]
123	ADLERQEKEVDEDTT	ubiquitination	[3]
161	ECEPGMVKGIHHGPS	ubiquitination	[3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. 

Sequence Annotation

 REGION 1 131 Interaction with the N-terminus of
 REGION 1 69 Interaction with the NDC80-CDCA1
 REGION 132 197 Interaction with the C-terminus of
 MOD_RES 11 11 Phosphoserine.  

Keyword

 3D-structure; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 197 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031262; C:Ndc80 complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 

Interpro

 IPR013252; Ndc80_Spc24. 

Pfam

 PF08286; Spc24 

SMART

  

PROSITE

  

PRINTS