UniProt Accession

 HDHA_ECOLI; P0AET8; P25529 

Genbank Protein ID

 D10497; U00096; AP009048; M14641 

Genbank Nucleotide ID

 BAA01384.1; AAC74691.1; BAA15370.1; AAA68921.1 

Protein Name

 7-alpha-hydroxysteroid dehydrogenase 

Protein Synonyms/Alias

 7-alpha-HSDH 

Gene Name

 hdhA 

Gene Synonyms/Alias

 hsdH; b1619; JW1611 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
85	LADFAISKLGKVDIL	acetylation	[1]
102	NAGGGGPKPFDMPMA	acetylation	[1, 2]
163	MTSYASSKAAASHLV	acetylation	[1, 2]
180	MAFDLGEKNIRVNGI	acetylation	[1]
198	AILTDALKSVITPEI	acetylation	[1]
208	ITPEIEQKMLQHTPI	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 7-alpha-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid. 

Sequence Annotation

 NP_BIND 18 42 NAD.
 ACT_SITE 159 159 Proton acceptor.
 BINDING 146 146 Substrate.
 BINDING 163 163 NAD.  

Keyword

 3D-structure; Bile acid catabolism; Complete proteome; Direct protein sequencing; Lipid degradation; Lipid metabolism; NAD; Oxidoreductase; Reference proteome; Steroid metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 255 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0008709; F:cholate 7-alpha-dehydrogenase activity; IDA:EcoCyc.
 GO:0030573; P:bile acid catabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.