UniProt Accession

 ML12B_HUMAN; O14950; D3DUH6; Q13182; Q7Z5Z4 

Genbank Protein ID

 D82058; AB046614; D50372; U26162; AY320408; AK291726; CH471113; CH471113; CH471113; BC004994 

Genbank Nucleotide ID

 BAB88918.1; BAB62403.1; BAA23323.1; AAA67367.1; AAP73808.1; BAF84415.1; EAX01675.1; EAX01676.1; EAX01677.1; AAH04994.1 

Protein Name

 Myosin regulatory light chain 12B 

Protein Synonyms/Alias

 MLC-2A; MLC-2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2; SHUJUN-1 

Gene Name

 MYL12B 

Gene Synonyms/Alias

 MRLC2; MYLC2B 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
35	QSQIQEFKEAFNMID	ubiquitination	[1]
51	NRDGFIDKEDLHDML	ubiquitination	[1, 2, 3, 4, 5]
150	YREAPIDKKGNFNYI	ubiquitination	[1, 2, 3, 4, 5]
151	REAPIDKKGNFNYIE	ubiquitination	[1, 2, 3, 4, 5, 6]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity). 

Sequence Annotation

 DOMAIN 29 64 EF-hand 1.
 DOMAIN 98 133 EF-hand 2.
 DOMAIN 134 169 EF-hand 3.
 MOD_RES 19 19 Phosphothreonine; by MLCK and ZIPK/DAPK3.
 MOD_RES 20 20 Phosphoserine; by MLCK and ZIPK/DAPK3.  

Keyword

 Calcium; Complete proteome; Metal-binding; Motor protein; Muscle protein; Myosin; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 172 AA 

Protein Sequence

Gene Ontology

 GO:0045177; C:apical part of cell; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016460; C:myosin II complex; IEA:Compara.
 GO:0001725; C:stress fiber; IEA:Compara.
 GO:0030018; C:Z disc; IEA:Compara.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0006936; P:muscle contraction; TAS:Reactome.
 GO:0008360; P:regulation of cell shape; IEA:Compara. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS